IFN-α对人脐带间充质干细胞粘附分子表达的影响

Effects of IFN-alpha with Different Concentration to Adhesion Molecules Expression in Human Umbilical Cord Mesenchymal Stem Cells

目的:本研究旨在观察不同浓度IFN-α对体外培养人脐带间充质干细胞表面粘附分子表达变化的影响。方法:采用组织块移行法培养人脐带间充质干细胞(Human umbilical cord mesenchymal stem cells,hucMSCs),并进行干细胞表面抗原、成骨和成脂鉴定。向P3代hucMSCs加入不同浓度的IFN-α,24小时后收集细胞,应用流式细胞仪检测CD44、CD49d、CD54、CD58、CD62p、CD62L、CD102及CD106等八种粘附分子的表达情况。结果:①生理状态下,CD106、CD62P、CD62L和CD102阳性表达率极低(均<1%),CD54表达最高,为41.58%,经IFN-α干预后,CD102、CD106、CD62L、CD62p阳性表达率略有升高,但总体变化不明显(均<5%)。②CD49d、CD54、CD58阳性表达率与IFN-α呈浓度依赖性,最高达(66.36±2.48)%、(76.26±1.85)%、(47.78±0.44)%;CD44在浓度为3×103U/ml时阳性表达率最高,为(49.81±3.25)%,且干预组与对照组、各组间比较差异有显著性意义(P<0.05)。结论:炎症因子IFN-α可显著提高hucMSCs表面CD54、CD58、CD44、CD49d的阳性表达率,但对CD102、CD106、CD62P和CD62L作用不明显。

Objective： To investigate the effects of IFN-（x with different concentration to adhesion molecules expression in mesenchymal stem cells derived from human umbilical cord tissue. Methods： The hucMSCs were isolated from human umbilical cord by tissue culture. The expressions of specific markers in hucMSCs were detected in the physical condition by flow cytometry. The adipogen- ic and osteogenic induction ofhucMSCs were detected by alizarin and Oil red O staining.hucMSCs were exposed to IFN-et of different concentration for 24h; analyses of expression of CD44, CD49d, CD54, CD58, CD62p, CD62L, CD102 and CD106 on cell surface were performed using flow eytometry. Results： In physiological state, hucMSCs express extremely low level of CD102, CD106, CD62P, CD62L while the expression of CD54 was relatively high, 41.58%. Stimulated by the 1FN-oL, the expression of CD102, CD106, CD62P, CD62L was increased slightly, but still below 5%, while CD49d, CD54, CD58 was upregulated concentration-dependently, up to （ 66.36＋ 2.48 ）%, （ 76.26＋ 1.85 ）%, （47.78＋ 0.44）% respectively; When exposed to IFN-a of 3 x 102 U/ml, CD44 could be increased to （49.81 ＋ 3.25 ）%. Conclusion： IFN-cx can elevate signaficatly the expression of CD54, CD49d, CD44 and CD58, but not significant for CD102, CD106, CD62P and CD62L on the surface of mesenchymal stem cells derived from human umbilical cord.