血清S100A12蛋白与克罗恩病活动性的相关研究

Correlation between serum S100A12 and activity of Crohn's disease

目的:研究血清S100A12蛋白水平在克罗恩病(CD)患者中的表达及其与CD活动性的关系。方法:选取44例确诊为CD的患者为研究对象,计算CD活动指数(CDAI),按照CDAI分为活动期CD患者和缓解期CD患者。同时选取16例非消化道感染的患者作为感染对照组,15名健康体检者作为正常对照组。用酶联免疫吸附法测定血清S100A12蛋白浓度。结果:CD患者血清S100A12蛋白平均浓度为875.85(575.58～1 259.9)ng/mL,明显高于正常对照组的485.3(349.2～571.2)ng/mL(Z=-4.11,P<0.01)。活动期和缓解期CD患者血清S100A12蛋白平均浓度分别为1 932(1792.4～2 202.5)ng/mL和600(460～712.5)ng/mL。活动期CD患者血清S100A12蛋白浓度明显高于缓解期CD患者(Z=-3.20,P<0.01)。CD患者血清S100A12蛋白浓度与CDAI呈线性相关(r=0.38,P<0.05)。结论:S100A12蛋白在CD的发病机制中起作用。血清S100A12蛋白水平与CDAI相关,可以作为疾病活动度的新指标。

Objective To study the correlation between serum S100A12 and activity of Crohn＇s disease （CD）. Methods Forty-four patients diagnosed as CD were selected and divided into active CD group and remission CD group according to CD activity index （CDAI）. Sixteen non-gastrointestinal infected patients were selected as infection control group and fifteen healthy people were recruited as healthy control group. Serum SIOOA12 level was determined by sandwich enzyme-linked immunosorbent assay （ELISA）. Results The median level of serum S100A12 in CD patients was 875.85 ng/mL （ranged from 575.58 ng/mL to 1 259.9 ng/mL）, which was significantly higher than those of healthy controls （485.3 ng/mL, ranged from 349.2 ng/mL to 571.2 ng/mL） （Z=-4.11, P〈0.01）. Serum S100A12 levels in active CD and remission CD groups were 1 932 ng/mL （ranged from 1 792.4 ng/mL to 2 202.5 ng/mL） and 600 ng/mL （ranged from 460 ng/ mL to 712.5 ng/mL）, respectively. The difference in serum S100A12 level between active and remission CD patients was statistically significant （Z=-3.20, P〈0.01）. There was a positive correlation between serum S100A12 level and CDAI （r= 0.38,P〈0.05） Conclusions Serum S100A12 level is up-regulated in CD patients, suggesting it plays an important role in the pathogenesis of CD. Serum S100A12 level correlates with CDAI, which may be served as a useful marker of disease activity in patients with CD.