摘要
目的:探讨白细胞介素-1β(IL-1β)对人牙周膜成纤维细胞(hPDLFs)中单核细胞趋化蛋白-1(MCP-1)表达水平的影响。方法:体外分离培养的人牙周膜成纤维细胞随机分为实验组和对照组,实验组细胞以不同浓度IL-1β(1、5、10、25 ng/mL)作用24 h;对照组细胞则不加IL-1β作用。分别采用半定量逆转录聚合酶链反应(RT-PCR)检测人牙周膜成纤维细胞中MCP-1 mRNA的表达;采用免疫荧光技术观察MCP-1的表达情况;采用Western blot方法检测其蛋白表达变化。结果:对照组hPDLFs中可见微弱的MCP-1表达;实验组hPDLFs经IL-1β刺激后,MCP-1在mRNA和蛋白水平表达都增强,这种调节作用在10 ng/mL IL-1β的作用浓度时最明显(P<0.05)。结论:在IL-1β介导环境下,hPDLFs中MCP-1的表达增高,而MCP-1表达增高可能是引起外周血单核细胞向局部炎症牙周组织募集的机制之一。
AIM： To investigate the effects of IL-1β on the expression of monocyte chemoattractant protein -1 （MCP-1） in human periodontal ligament fibroblasts （hPDLFs）. MEDHODS： hPDLFs were isolated and cultured in vitro, and then were randomly assigned to the experimental group and the control group. In the experimental group, hPDLFs were cultured in media containing various concentrations of IL-1β. In the control group, hPDLFs were cultured in media without IL-1β. After 24h incubation, semi-quantitative RT-PCR, immunofluorescence and Western- blot were performed to detect the expression of MCP-1 mRNA and protein. RESULTS： The expression of MCP-I was detected at low level in control hPDLFs. After pretreatment with IL-1β, the mRNA and protein expression of MCP - 1 were significantly enhanced, with 10ng/mL IL-1β showing the strongest stimulating effect. CONCLUSION： The expression of MCP-1 in hPDLFs could be induced by IL-1β and might enhance the chemoattraction of monocytes from peripheral blood toward the inflammatory sites of periodontium.
出处
《牙体牙髓牙周病学杂志》
CAS
北大核心
2012年第11期619-622,632,共5页
Chinese Journal of Conservative Dentistry
基金
国家自然科学基金资助项目(81070870)
