增殖细胞核抗原在卵巢上皮性癌的表达及其临床意义

Expression and clinical significance of proliferating cell nuclear antigen in patients with ovarian epithelial carcinoma

目的 :探讨增殖细胞核抗原 (PCNA)表达与卵巢上皮性癌生物学行为及预后的关系。材料和方法 :采用LSAB免疫组化方法检测 84例石蜡包埋的卵巢上皮性癌组织中增殖细胞核抗原的表达 ,分析其表达与患者的年龄、临床分期、组织分化程度、组织学类型及预后之间的关系。结果 :PCNA在卵巢上皮性癌的表达率为 10 0 % ,表达强度与患者的年龄呈正相关 (P <0 0 5 ) ,与卵巢上皮性癌的组织分化程度呈负相关 (P <0 0 1) ,而与临床分期、组织学类型之间无关 ;单因素分析显示PCNA的表达与卵巢上皮性癌的预后呈负相关 (P <0 0 5 ) ,但多因素分析未能显示这种相关性。结论 :PCNA在卵巢上皮性癌的阳性表达率高 ,组织分化程度越低 ,年龄越大 ,PCNA表达强度越强 ;PCNA并不是估计卵巢癌预后的有效指标 ;临床分期。

Objective:To investigate the relationship between the expressions of proliferating cell nuclear antigen (PCNA) and the clinicobiological characteristics and prognosis in ovarian epithelial carcinomaMethods:84 specimens of ovarian epithelial carcinoma were detected for the expressions of PCNA by using labellingstreptavidinbiotin(LSAB) immunohistochemistry assay, and the correlations were analyzed for the PCNA expression with stage, histological grade, and histologic subtype of the tumor, and age and prgnosis ofthe patientResults:All tumors had PCNA expression, the positive rate was 100%There were significant correlations between the expressional grade of PCNA and the age (P< 005 ), and histological grade ( P< 001 )No significant correlation was found between the expressional grade of PCNA and stage or histologic subtype ( P > 005 )In unvariate analysis, the expression of PCNA was associated with poor survival in ovarian epithelial carcinoma, but it did not retainany prognostic significance in multivariate analysisConclusions:PCNA expressions in ovarian epithelial carcinoma is high The tumor with lower histological grade, or in the patients of older age, will express higher level of PCNAThe expression of PCNA is not an independent prognostic factor for prognosis in epithelial ovarian carcinomaStage and histological grade are still the significant prognostic factors in the epithelial ovarian cancer