摘要
目的研究错配修复基因hMLH1和干细胞标志物CD44在胃癌的表达状况、意义及相互间的关系。方法采用免疫组化SP法检测79例胃癌组织和相对应的癌旁组织中错配修复基因hMLH1和干细胞标志物CD44的表达状况,结合临床资料分析这两个指标的表达与肿瘤病理参数间的关系,运用卡方检验分析该两个指标间的相互关系。结果79例胃癌标本中,hMLH1阳性表达的有15例,占19%,,CD44阳性表达的有53例,占67.1%,两者在癌旁正常组织表达的阳性率分别为51%和15.2%,两者差异均有统计学意义(P<0.05);胃癌组织中hMLH1的表达与CD44呈负相关(P<0.05);hMLH1阴性表达、CD44阳性表达与胃癌淋巴结转移、浸润深度、TNM分期及脉管浸润呈正相关(P<0.05)。结论 hMLH1在胃癌的形成过程中起到重要作用,它与胃癌干细胞标志物CD44之间存在拮抗关系。
Objective To explore the significance of expressions of hMLH1 and CD44 in human gastric cancer and their relationship. Methods The expression status of hMLH1 and CD44 in 79 gastric cancer tissues was examined by immunohistochem- istry and corresponding adjacent tissues and its clinical significance. Results The positive expression of hMLH1 in two groups （gas- tric carcinoma, adjacent gastric mueosa） were 19%, 51% respectively, with the difference between two groups being statistically significant（P〈0.01 ）; The rates of CD44 were 67.1%, 15.2% in two groups （P〈0.01） respectively. The rate of hMLH1 in the sub- group with lymphatic metastasis, heavy invasive depth, high disease stage, vessel invasion was higher than that in the group with no lymphatic metastasis, mild invasive depth, low disease stage, less vessel invasion. The positive rate of hMLH1 expression was nega- tive correlated with CD44 in gastric carcinoma（r=0.488, P〈0.01 ）. Conclusion hMLH1 play an important role in gastric earcino- zenesis and it is inversely related to CD44.
出处
《解剖学研究》
CAS
2012年第5期343-346,共4页
Anatomy Research
基金
湖北省教育厅重点项目(D200528005)
湖北省教育厅B类指导性项目(B20112806)
