转人乳铁蛋白基因大米的慢性毒性研究

Study on chronic toxicity of genetically modified rice expressing human lactoferrin

目的观察转人乳铁蛋白(hLF)基因大米是否具有慢性毒性作用。方法初断乳的180只SD大鼠按性别、体重随机分为3组:转基因hLF基因大米组、亲本大米对照组和AIN-93对照组,分别饲喂相应饲料12个月。观察大鼠的体重、进食量、血常规、血生化情况,实验末期处死动物,称量脏器重量并对脏器进行病理学检查。结果转hLF基因大米组血常规(LYM%、GRN%)、血生化(ALT、AST、GLU)在个别时间点上与亲本对照组或AIN-93对照组存在显著差异(P<0.05);其他观察指标与两个对照组均无显著差异。结论现有实验结果不能证实转hLF基因大米对大鼠有慢性毒性作用。

Objective To observe the chronic toxic effect of the genetically modified rice which harboring human lactoferrin（hLF） gene on rats.Methods Based on gender and weight,180 weanling SD rats were randomly divided into three groups： transgenic rice group,parental rice group and AIN-93 normal control group.They were fed for 12 months.Body weight,food consumption,hematology and clinical biochemistry were observed during the experiment period.The rats were sacrificed at the end point for organ weight measurement and pathological examination.Results The differences of some indices of hematology（LYM%,GRN%） and clinical biochemistry（ALT,AST,GLU） between hLF rice group,parental rice group and AIN-93 group were significant（P0.05） while no other significant difference was observed.Conclusion There was inadequate evidence to indicate the hLF rice had adverse effects on rats.