成骨细胞培养及冻存复苏后生物学特性比较

A comparison study on biologic phenotypes of cultured osteoblasts and which after cryopreservation

目的 :了解冻存复苏过程对保持成骨细胞生物学特性的影响。方法 :通过细胞组化、免疫组化以及图像分析等方法对冻存和非冻存 (新鲜制备 )的细胞在其形态及功能方面进行了比较。结果 :两组ALP染色、茜素红S法、V G法、免疫组化法的结果均差异无显著性 (P >0 0 5 )。但细胞存活率与冻存时间密切相关 ;随冻存时间延长 ,其存活率下降。结论 :两组细胞保持了某些相似的生物学行为 ,如细胞形态、生长过程、基质分泌等。冻存时间对保存的成骨细胞存活率有影响。

Purpose To study the effects of biological characters of osteoblasts after cryopreservation and resuscitation. Method The compared studies were done in cultured osteoblasts and those after cryopreservation by histochemistry, immunohistochemistry and image analysis in cytomorphology and functions. Results The results showed that there were no differences between two groups in ALP staining, alizarin red S staining, Van Gieson staining and immunohistochemistry(P>0 05). But the cell survival rate was related to the time after cryopreservation. As the time after cryopreservation went on, its survival rate was declining. Conclusion Two groups had some similar biological behaviors in cytomorphology, growth process and matrix secrete etc. The time after cryopreservation affected the survival rate of osteoblasts after cryopreservation.