MMP-9、CD147在涎腺腺样囊性癌中的表达及临床意义

Expression and Clinical Significance of MMP-9 and CD147 in Salivary Adenoid Cystic Carcinoma

目的:联合检测人涎腺腺样囊性癌组织中MMP-9、CD147的表达并分析其相关性,探讨其与临床病理参数间的关系,为腺样囊性癌的临床治疗和预后判断提供理论依据。方法:选择山西医科大学第一医院病理科存档的腺样囊性癌组织标本21例(癌组),正常涎腺组织6例(对照组)。21例腺样囊性癌分别依据临床分期、有无侵犯神经进行分组。运用免疫组织化学PV-9000二步法检测MMP-9、CD147,结果用SPSS 13.0软件分析。结果:MMP-9及CD147在腺样囊性癌组中的阳性表达率(分别为76.2%和81.0%)明显高于在对照组中的阳性表达率(分别为16.7%和16.7%),差异有统计学意义(分别为P<0.05和P<0.01)。Ⅲ+Ⅳ期腺样囊性癌病例MMP-9及CD147的阳性表达率(均为100.0%),明显高于Ⅰ+Ⅱ期病例(分别为44.4%和55.6%),差异有统计学意义(分别为P<0.01和P<0.05)。有无侵犯神经的病例组间比较,MMP-9及CD147的表达差异没有统计学意义(P>0.05)。MMP-9和CD147在腺样囊性癌组织中均呈阴性、弱阳性、阳性、强阳性表达者分别为4、5、7和3例,表达一致率为90.5%,Kappa值为0.870。结论:MMP-9和CD147均可作为反映腺样囊性癌细胞生物学行为的客观参考指标,其表达与临床分期密切相关,且二者之间的表达有正相关。

Objective： To investigate the expression of matrix metalloproteinase （MMP）-9 and CD147 in human salivary adenoid cystic carcinoma （SACC） tissues and their correlations. Methods： 21 cases of human SACC tissues and 6 cases of normal human salivary tissues were examined by immunohistochemical method. The relationship between the expression and clinical-pathological behaviors was also analyzed. Follow-up data were statistically analyzed. Results： The expression of MMP-9 and CD147 in SACC tissues was higher than those in normal salivary tissues. Positive expression rate of MMP-9 and CD147 in SACC tissues was 76.2% and 81.0% respectively, significantly higher than those of normal salivary tissues （P〈O.05, P〈O.01 respectively）. The staining intensity of MMP-9 and CD147 in SACC tissues was closely associated with the clinicopathologic stage （P〈0.01, P〈0.05 respectively）, but no distinct difference was found in other clinical parameters. There was also a significant correlation in staining intensity between MMP-9 and CD147. The co- expression rate of MMP-9 and CD147 in SACC was 90.5%. Conclusion： （1） MMP-9 and CD147 may be an objective marker to estimate the behaviors of SACC. （2）The expression of MMP-9 and CD147 in SACC tissues correlates with clinical stage. （3） There is a significant positive correlation between the expression of MMP-9 and CDl47.