膀胱癌患者血清DKK-1和DKK-3表达及其临床意义

Serum DKK-1 and DKK-3 expressions in patients with bladder cancer and their clinical significance

目的:研究血清Dickko pf(DKK)-1和DKK-3蛋白在膀胱癌患者术前、术后及正常人中的表达情况,并进一步探讨其与膀胱癌临床病理特征之间的关系。方法:应用ELISA法检测40例膀胱癌患者术前及术后外周血清中DKK-1和DKK-3的表达情况,并与20例非肿瘤对照组患者相比较。相关性分析DKK-1和DKK-3水平之间的相关性,以及与患者临床病理特征的关系。结果:膀胱癌患者术前血清中DKK-1浓度明显高于术后及对照组患者;血清DKK-3浓度在膀胱癌患者术前和术后无明显改变,但术前、术后血清DKK-3浓度均明显低于对照组。DKK-1浓度与膀胱癌患者的临床病理特征不相关,DKK-3在高级别尿路上皮癌中的表达明显高于低级别尿路上皮癌。另外,DKK-1和DKK-3表达之间无相关性。结论:血清DKK-1和DKK-3水平与膀胱癌的发生关系密切,其中DKK-1有可能成为一种新的肿瘤标志物,而DKK-3下调可能参与了膀胱癌的浸润及转移。提示测定两蛋白表达可能有助于膀胱癌的诊断和术后预测。

Objective： To investigate the expressions of serum DKK （Dickkopf）-1 and DKK-3 in patients with bladder cancer before and after surgical operation and the patients with ureteral calculi and urethral caruncles, and to explore the correlations of their expressions with the clinicopathological features. Methods： Serum DKK-1 and DKK-3 levels in 40 patients with bladder cancer before and after surgical operation were detected by enzyme-linked immunosorbent assay and compared with those in 20 patients with ureteral calculi and urethral caruncles. Spearman＇s correlation analysis was used to evaluate the intercorrelation of DKK-1 and DKK-3 expressions and their relationship with clinicopathological characteristics. Results： Serum DKK-1 level in preoperative patients with bladder cancer was significantly higher than those in postoperative bladder cancer patients and patients with ureteral calculi and urethral caruncles. Serum DKK-3 level had no significant change between preoperative and postoperative patients with bladder cancer, but it was significantly lower in patients with bladder cancer than the patients with ureteral calculi and urethral caruncles. Serum DKK-1 level was not associated with clinicopathological characteristics, but serum DKK-3 level was significantly higher in the high-grade urothelial carcinoma than that in the low-grade urothelial carcinoma. Conclusion： Serum DKK-1 and DKK-3 expressions are closely related with the occurrence of bladder cancer. DKK-1 may become a new tumor marker. The downregulation of DKK-3 may be involved in the invasion and metastasis of bladder cancer. These results suggest that the detection of Serum DKK-1 and DKK-3 may contribute to the diagnosis and postoperative prediction of bladder cancer.