血红蛋白在溶胶-凝胶纳米银修饰电极上的直接电化学

Direct Electrochemistry of Hemoglobin Immobilized on Modified AgNPs Glassy Carbon Electrode

运用溶胶-凝胶技术将血红蛋白(Hb)固定于纳米银(AgNPs)修饰的玻碳电极(GCE)表面,制得溶胶-凝胶血红蛋白纳米银修饰电极(Sol-Gel/Hb/AgNPs/GCE)。优化了修饰电极的制备条件,研究了该修饰电极在B-R缓冲溶液(pH=4.10)中的电化学行为,探讨了Hb在AgNPs修饰电极表面的直接电子转移机理。结果表明:AgNPs不仅保持了Hb的生物活性,而且通过它的催化效应,实现了Hb与电极之间的直接电子转移。进一步的实验结果显示,固定在纳米银修饰电极表面的Hb能保持其对H2O2的生物电催化活性。

In this paper,hemoglobin （Hb） was modified on the surface of a glassy carbon（GC） electrode by Sol-Gel technology, and a steady Sol-Gel/Hb/AgNPs/GCE was obtained. The electrochemistry behavior of the Sol-Gel/Hb/AgNPs/GCE biosensor in B-R（pH = 4. 1）and the direct electron transfer mechanism of Hb were discussed,indicating that silver nanoparticles （AgNPs）well kept the biological activity of Hb. Moreover through alkali effect of silver nanoparticles,the direct electron transfer between Hb and the electrode was realized. The experimental results also demonstrated that the immobilized Hb maintained its bioelectrocatalytic activity toward H2O2.