肿瘤坏死因子-α及其基因863C—A多态性与壮族甲状腺相关眼病的相关性研究

Correlation research between Gene Polymorphism of TNF-alpha Gene 863C_A and Thyroid Associated Ophtalmopathy in Zhuang Ethnic Population

目的 探讨肿瘤坏死因子-α（TNF-α）及其基因863C_A多态性与壮族甲状腺相关眼病（TAO）的相关性。方法 选择壮族TAO患者与汉族TAO患者作为研究对象,并分别选择健康壮族人与汉族人设立对照。抽取患者血液制备血清及DNA样本,分别采用酶联免疫吸附法测定血清TNF-α水平,采用聚合酶链反应（PCR）法检测TNF-α 863C_A位点基因多态性。结果 与对照组比较,TAO患者血清TNF-α水平显著升高（P〈0.05）,壮族TAO患者升高更为显著（P〈0.05）;壮族人群TAO患者CA基因型频率（36.9%）显著高于汉族人群TAO患者（22.3%）,AA基因型频率（19.6%）显著低于汉族人群TAO患者（21.3%）（χ2=0.770、2.145,均P〈0.05）。结论 TAO发病时血清TNF-α水平显著升高,在汉族与壮族之间存在差异,可能由于基因多态性引起。

Objective To explore correlation between gene polymorphism of TNF-alpha and thyroid associated ophtalmopa thy （TAO） in Zhuang ethnic population.Methods DNA and serum samples were collected in patients with TAO in Han/Zhuang population.ELISA was applied to detect level of TNF-alpha in serum and PCR was applied to detect polymorphism of TNF-alpha gene 863C_A.Results The levels of TNF-alpha in patients with TAO increased significantly compared with control group（P〈0.05）,and more significantly in Zhuang population compared with Han population（P〈0.05）;gene frequency of CA was significantly higher in patients with TAO in Zhuang population than that in Han population（P〈0.05）,while gene frequency of AA was significantly lower（χ2=0.770,2.145,all P〈0.05）. Conclusion Serum level of TNF-alpha increases during occurrence of TAO,and the differences of expression level between different nationalities are due to gene polymorphism of TNF-alpha.