津力达颗粒对糖尿病大鼠海马组织的保护作用

Protective effect of Jinlida granules on hippocampus of diabetic rats

目的探讨津力达颗粒对糖尿病大鼠海马组织的保护作用。方法采用高脂饮食、链脲佐菌素腹腔一次性注射诱发SD大鼠糖尿病模型,随机分为模型组、津力达不同剂量(0.75、1.5、3.0 g/kg)治疗组、α-硫辛酸组、胰岛素组,另设正常对照组。8周后,通过Morris水迷宫实验测试大鼠学习记忆能力,然后处死大鼠取海马组织,采用TUNEL法检测凋亡细胞,透射电镜下观察超微结构,并测定海马组织中超氧化物歧化酶(SOD)、谷胱甘肽(GSH)、髓过氧化物酶(MPO)活性和丙二醛(MDA)含量。结果糖尿病大鼠成模后8周出现学习记忆功能障碍,除低剂量津力达组外,各药物治疗组的学习记忆功能均有不同程度的改善。与正常对照组比较,模型组大鼠海马CA1区出现较多的凋亡细胞,超微结构明显破坏,SOD、GSH活性降低(P<0.05),MPO活性和MDA水平升高(P<0.05)。与模型组相比,各药物治疗组大鼠海马CA1区凋亡细胞均有不同程度的减少,海马神经元的超微结构破坏程度均有不同程度的减轻;除津力达低、中剂量组外,其余各药物治疗组SOD、GSH活性均升高(P<0.05),MPO活性均降低(P<0.05),其中津力达高剂量组与α-硫辛酸组效果相当。结论津力达颗粒对糖尿病大鼠海马组织具有保护作用。

Objective To investigate the protective effect of Jinlida granules on hippocampus of diabetic rats. Methods Diabetic models were induced by high-fat diet and intraperitoneal injection of streptozotocin（STZ） in SD rats. The study was divided into diabetic model group, Jinlida granule groups（0.75, 1.5 ,and 3.0 g/kg）, a-lipoic acid group and insulin group. Healthy rats served as normal controls. Rats were tested in Morris water maze after 8 weeks of treatment, and then the hippocampus tissues were taken from each group and were subjected to TUNEL assay and transmission electron microscopy （TEM） observation. The activities of superoxide dismutase （SOD）, glutathione （GSH）, myeloperoxidase （MPO）, and the contents of malondialdehyde （MDA） were all examined. Results The diabetic rats developed studying and memory disorders 8 weeks after establishment. Except for those in the low-dose Jinlida group, rats in other treatment groups had improved studying and memory functions to different extents. Compared with normal control group, rats in the model group had more apoptotic neurons in the hippocampal CA1 area, prominent ultrastructure damage, significantly decreased SOD and GSH activities （P〈0.05）, and significantly increased MPO activity and MDA level （P〈0.05）. Compared with the model group, rats in all the treatment groups had decreased apoptosis and less ultrastructure damage;and rats in high-dose Jinlida group, a- lipoic acid group and insulin group had significantly increased SOD and GSH activities and significantly decreased MPO activity in the hippocampal CA1 area （P〉0.05） ; and the high-dose Jinlida granule group had a similar effect to a-lipoic acid group. Conclusion Jinlida granule can protect the hippocampus of diabetic rats.