摘要
目的观察慢性精神应激对雌性大鼠卵巢储备功能的影响。方法性成熟的雌性Wistar大鼠24只,随机分为实验组及对照组。实验组采用慢性温和不可预知应激(CUMS)法,模拟慢性精神应激,引起大鼠情绪改变;对照组不给予任何干预,光照规律,食水随意,安静饲养。观察大鼠血清中雌二醇(E_2)、促卵泡生成素(FSH)的表达水平,以及卵巢组织病理形态变化。结果与对照组比较,实验组大鼠体质量增加较少,活动减少,糖水偏好度下降,动情次数减少,差异有统计学意义(P<0.05),血清E_2水平降低,FSH水平升高,差异亦有统计学意义(P<0.05)。实验组大鼠卵巢中各级卵泡数、黄体数、卵泡液中颗粒细胞层数较对照组明显减少,并伴有不同程度血管扩张。结论慢性精神应激可导致雌性大鼠卵巢储备功能下降。
Objective To observe the impact of chronic mental stress on ovarian reserve function in female rats. Methods Sexually mature female Wistar rats were randomly divided into experimental and control groups.Expression of the chronic mild unpredictable stress（CUMS） method,the simulation of chronic mental stress was used to cause rats mood changes, observed in serum estradiol（E,）,follicle stimulating hormone（FSH） level,as well as ovarian tissue pathological changes. Results Serum E_2 levels in experimental group decreased,and FSH levels increased,compared with the control group, there were significant differences between them（P 0.05）.While in the experimental group,all levels of the number of follicles in the ovary,the number of luteal,follicular fluid,granulosa cell layers was significantly reduced than in the control group,and with varying degrees of blood vessels to dilate.Conclusion Chronic mental stress can lead to decrease of ovarian reserve in female rats.
出处
《疑难病杂志》
CAS
2013年第2期124-126,F0003,共4页
Chinese Journal of Difficult and Complicated Cases
基金
北京中医药大学自主选题资助项目(No.2011YJBZZXS-029)
