硫酸长春新碱固体脂质纳米粒的制备及其性质考察

Preparation and Characterization of Vincristine Sulfate-loaded Solid Lipid Nanoparticles

目的以单硬脂酸甘油酯为载体材料,采用复乳溶剂挥发法制备硫酸长春新碱固体脂质纳米粒(VCR-SLN),并考察其理化性质。方法采用复乳溶剂挥发法制备VCR-SLN,以正交设计优化处方及制备工艺,并考察其形态、粒径、Zeta电位、包封率、载药量和体外释放。结果 VCR-SLN为类球形实体粒子,平均粒径为(144.83±2.71)nm,Zeta电位为(-24.77±0.513)mV,包封率为(40.54±0.45)%,载药量为(1.14±0.074)%。药物体外释放曲线符合Weibull方程。结论复乳溶剂挥发法适用于制备硫酸长春新碱固体脂质纳米粒。

OBJECTIVE To prepare vincristine sulfate-solid lipid nanoparticles（VCR-SLN） with single stearic acid glyceride as its lipid matrix and investigate its characterization. METHODS Multiple emulsion-solvent diffusion technique was adopted to prepare VCR-SLN and the orthogonal design was applied to optimize its formula and technology. Its morphology, particle size, ζ potential, drug entrapment efficiency, drug loading capacity and in vitro release of the VCR-SLN were investigated. RESULTS VCR-SLN were spherelike and smooth, the mean particles size was （144.83±2.71）nm, ζ potential was （-24.77±0.513）mV, entrapment efficiency was （40.54±0.45）%, drug loading capacity was （1.14±0.074）%. The release profile of vincristine sulfate in vitro fitted Weibull equation. CONCLUSION VCR-SLN can be prepared very well by multiple emulsion-solvent diffusion technique.