喉癌微血管生成中P53 nm23蛋白和血管内皮生长因子的作用

Role of P53,nm23 proteins and vascular endothelial growth factor in angiogenesis and metastasis of laryngeal cancer

目的 探讨P5 3、nm2 3蛋白和血管内皮生长因子 (vascularendothelialgrowthfacor,VEGF)在喉癌微血管生成及转移中的作用。方法 通过免疫组化SP法对 42例喉癌标本中P5 3、nm2 3蛋白、VEGF及微血管密度 (microvesseldensity,MVD)进行了检测。结果 喉癌中P5 3、nm2 3蛋白及VEGF的阳性表达率分别占 47 6 %、5 7 1%和 71 4%。P5 3基因和VEGF呈正相关 (P <0 0 5 ) ,在有淋巴结转移的喉癌标本中P5 3、VEGF阳性表达率及MVD明显高于非转移组 (P <0 0 5 )。而nm2 3基因和VEGF在喉癌标本中无直接相关性 ,在nm2 3蛋白表达阴性和VEGF阳性标本中MVD较高 ,这种现象多见于有淋巴结转移的喉癌中。结论 突变型P5 3基因通过调控VEGF的表达影响肿瘤内MVD ,促使喉癌发生转移 ;而nm2 3基因可能不是通过调控VEGF的表达 ,而是通过其它途径影响喉癌转移。

Objective To elucidate the role of P53, nm23 proteins and vascular endothelial growth factor (VEGF) on angiogenesis and metastasis of laryngeal cancer Methods Specimens of 42 laryngeal carcinomas were studied by immunohistochemical staining for P53 protein, nm23 protein, VEGF and CD34 antibody Results Positive expressions of P53 protein, nm23 protein and VEGF were found in 47 6%, 57 1% and 71 42% of laryngeal cancer specimens respectively There was a positive relationship between P53 and VEGF expression The microvessel density (MVD) in P53 or VEGF positive tumors was significantly higher in patients with cervical lymph node metastases than those without metastases No correlation was identified between nm23 and VEGF staining The MVD in nm23 negative and VEGF positive tumors was significantly high These phenomena were more easily found in patients with cervical lymph node metastases than in patients without metastases Conclusion P53 gene play roles in the metastasis of laryngeal cancer through regulating VEGF expression and reacting MVD in tumor; and nm23 gene reacts through approaches other than regulating VEGF expression