秦归活络颗粒中芍药苷的含量测定

Determination of Concentration of Paeoniflorin in Qinguihuoluo Granules

目的:建立一个稳定可靠的方法来测定秦归活络颗粒中芍药苷的含量。方法:本实验采用高效液相色谱法测定。色谱柱:YMCC18(4.6mm×150nm,5μm);流动相:乙腈-0.1%磷酸水溶液(15:85);流速为:1.0ml/min;检测波长为230nm,进样量为10μl。结果:芍药苷的保留时间为8.7分钟,在20.2μg/ml-101.0μg/ml范围内有良好的线性(r=0.99998,n=5);芍药苷的回收率为104.0%,RSD=0.7%(n=6);供试品放置24小时内稳定,RSD=0.61%(n=5)。结论:本方法测定结果准确,操作简便,重现性好,可用于秦归活络颗粒中芍药苷的含量测定。

Objective：To determine the concentration of paeoniflorin in Qinguihuoluo Granules by high performance liquid chromatography （HPLC） with UV detector. Methods：Internal standard method was used. The determination of paeoniflorin was carried out by YMC C18（4.6mm × 150nm,5μm） column with CH3CN -0. 1%H3PO4（15：85）as the mobile phase;the speed was 1.0ml/min;the detector wave length was 230nm;and the sample volume was 10μL Results：The Retention time of paeoni- florin was 8. 7 minute, and calibration curve for paeoniflorin was liner in the range of 20. 2μg/ml - 101.01a, g/ml （ r = 0. 99998,n =5）. the recovery rate of paeoniflorin was 104.0% ,RSD =0. 7%. And the drug was stable in 24 hours,RSD = 0. 61%. Conclusion ：This method was accurate for the determination of paeoniflorin in Qinguihuoluo Granules.