摘要
裂殖壶菌是一种理想的DHA的新生资源。其合成的DHA位于胞内,因此为了获得DHA,需要对裂殖壶菌细胞进行破碎,但是目前的破碎方法效率不高。本试验采用高压均质与酶法相结合的方法来破碎裂殖壶菌。结果表明在50 MPa下高压均质两次后,添加1.5%的碱性蛋白酶,在pH为10,温度为55℃条件下,酶解1.5 h后裂殖壶菌破碎率可达96.6%。因此,该复合法相比单一的高压均质破碎显著提高了裂殖壶菌破碎率。
Schizochytrium sp. is a kind of ideal new resources of DHA. It needs to break the cell of Schizochytrium sp. to obtain DHA because DHA is a product of the intracellular. But at present, the efficiency of the cell disruption methods is not so high. The method of high pressure homogenization combined with enzymatic disruption was discussed in the study. The results showed that the optimal condition to achieve the highest cell disruption rate was as follow; adding 1.5% alcalase, pH 10, temperature 55℃, enzymolysis time 1.5 h after 50 MPa twice homogenizing, and the cell disruption rate of Schizochytrium sp. could be up to 96.6%. Therefore, this combination method significantly increased the cell broken rate compared to single high oressure homogenization
出处
《食品工业》
北大核心
2013年第3期91-94,共4页
The Food Industry
关键词
裂殖壶菌
高压均质
酶法破碎
细胞破碎率
Schizochytrium sp.
high pressure homogenization
enzymatic disruption
cell disruption rate
