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异羟基洋地黄毒甙元(Digoxigenin)标记的探针在乙型肝炎病毒核酸杂交中的应用 被引量:6

USE OF DIGOXIGENIN LABELLED PROBE IN HEPATITIS B VIRUS DNA HYBRIDIZATION
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摘要 应用异羟基洋地黄毒甙元标记的探针,检测了人和鸭的血清及肝脏中的乙型肝炎病毒核酸,并与^(32)P标记的同位素探针做了比较。结果证明,该探针的特异性和敏感性与同位素探针一致(0.2pg)。它可用于各种核酸杂交试验,如打点杂交、Southern和Northern转印杂交试验等。恰当地从标本中提取待测核酸,是应用该探针的重要条件。 A DNA hybridization assay for human and duck hepatitis B virus in serum and liver specimens is described using a nonradioactive Digoxigenin labelled DNA probe, and compared with 32P probe. Results show that the sensitivity and specificity of both probes are the same ( 0.2pg ) . Satisfactory results were obtained by using Dig probe in dot blot, Southern and Northern blot. The main condition for the successful hybridization with Dig probe is the method for optimal extraction of target DNA from samples to be tested.
出处 《病毒学报》 CAS CSCD 北大核心 1991年第1期54-59,共6页 Chinese Journal of Virology
关键词 乙肝病毒 核酸杂交 DIGOXIGENIN Digoxigenin Nucleic acid hybridization assay Hepatitis B virus Duck hepatitis B virus
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  • 1Chan Y Y

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