泰山赤灵芝复合降解酶提取白花丹参须根有效成分的研究

Study on effective constituents extracted from fibrous roots of Salvia miltiorrhiza with degrading multi-enzymes from Taishan Ganoderma lucidum

目的:研究泰山赤灵芝复合降解酶在白花丹参须根有效成分提取中的应用。方法:采用木质纤维素复合降解酶来提取丹参须根中的有效成分,通过正交试验法优化酶解参数。结果:利用优化酶解工艺所得到的丹参须根提取液中总丹参酮和总丹酚酸的提取率可达11.923%,12.465%,比常规非酶法提取提高了62.794%,56.086%。结论:采用木质纤维素复合降解酶酶解法可以充分提取出丹参须根中的有效成分,为中药废弃物的再次利用提供了一条新途径。

Objective： To study the application of degrading multi-enzymes from C.anoderma lucidum in extracting effective constituents from fibrous roots of Salvia miltiorrhiza. Method： Effective constituents were extracted from fibrous roots by degrading multi-enzymes of wood fiber. The enzymatic parameters were optimized by the orthogonal design. Result： The extraction effieiencies of total tanshinones and total salvianolic acids in the extracts of fibrous roots of S. miltiorrhiza was obtained using optimum enzymolysis process reached 11. 923% , 12. 465% , respectively, which were 62. 794% , 56. 086% more than that by conventional non-enzymatic hydrolysis. Conclusion： Degrading muhi-enzymes of wood fiber can be used to fully extract effective constituents from fibrous roots of S. rniltiorrhiza, which provides a new approach for recycling wastes of traditional Chinese medicines.