血畅宁组方有效部位总皂苷HPLC特征图谱研究

Studies of HPLC Characteristic Chromatogram of Saponins in Effective Parts of Xuechangning Formula

目的:建立血畅宁组方有效部位总皂苷的HPLC特征图谱。方法:采用HPLC法,色谱条件Synergi Fusion-RP C18(4.6 mm×250 mm,5μm);流动相为乙腈-水溶液,梯度洗脱进行色谱分离;检测波长203 nm;流速1.0 mL.min-1;柱温(30±5)℃。结果:12批不同批次的血畅宁组方药材所得皂苷部位中,除3批外,其余9批与系统生成的对照特征图谱的相似度均在0.9以上;归纳出血畅宁组方皂苷部位有19个共有峰,不同批次样品中主要化学成分组成相似,但相对比例有明显的差异。结论:所建立的方法简单可行,能有效控制血畅宁组方有效部位总皂苷的质量。

Objective： To study the characteristic chromatogram of saponins in effective parts of Xuechangning formula by HPLC. Method： The chromatographic condition of HPLC was as follows： Synergi Fusion-RP column C18（4.6 mm×250 mm, 5 μm） at 30 ℃, the mobile phase being acetonitrile with water, gradient elution in chromatographic separation, the detective wavelength being 203 nm, and flow rate being 1.0 mL·min-1. Result： Except for three batches of 12 batches of saponins in Xuechangning formula, the characteristic chromatogram similarity value of the other eight batches was above 0.9 as compared with system generation spectrum, and 19 common characteristic peaks were presented.The results showed that the main chemical constituents of medicinal materials were similar among samples from different habitats, but their relative ratios are significantly different. Conclusion： The method is simple, accurate and reproducibility, and can be used for the quality control of Xuechangning formula.