当归芍药散对肝硬化腹水大鼠的干预作用研究

Study on intervention effect of Danggui Shaoyao San on rats with cirrhotic ascites

目的:研究当归芍药散对肝硬化腹水大鼠的干预作用,并从精氨酸加压素(AVP)途径探讨其对肝硬化腹水的影响。方法:雄性SD大鼠随机分为正常组、模型组、当归芍药散低、中、高剂量组(0.43,0.86,1.72 g.mL-1)。采用苯巴比妥联合CCl4法建立肝硬化腹水大鼠模型。收集24 h尿液,观察各组尿排泄情况;滤纸吸收法测定腹腔积液量;全自动生化仪检测血清丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST);全自动血液凝固分析仪检测血浆凝血酶原时间(PT);酶联免疫吸附测定法(ELISA)检测血浆AVP浓度。肝脏组织HE染色,观察组织形态变化。结果:当归芍药散给药组与模型组比较肝功能明显改善:血清ALT,AST水平显著降低,血浆PT明显缩短;肝脏病理组织形态均有不同程度改善;腹腔积液量均明显减少;24 h尿量显著增多;血浆AVP浓度显著降低。结论:当归芍药散可明显改善肝硬化腹水大鼠肝功能,减少腹水生成及延缓肝脏病理改变进程,其作用机制可能与精氨酸加压素有关。

Objective： To investigate the intervention effect of Danggui Shaoyao San on rats with cirrhotic ascites, and discuss the effect of arginine vasopressin （AVP） on cirrhotic ascites. Method： Male SD rats were randomly divided into the control group, the model group, Danggui Shaoyao San low, middle and high dose groups. The cirrhotic ascites rat model was established by CC14 com bined with phenobarbital. Their urines were collected at 24 h to observe urine excretion of each group. Filter papers were used to deter mine the amount of ascites. The levels of serum alanine aminotransferasa （ ALT）, aspartate aminotransferase （AST） were detected by the automatic biochemistry analyzer. Plasma prothrombin time （PT） was evaluated by the blood coagulation analyzer. The concentra tion of AVP in plasma was detected by enzymelinked immunosorbent assay （ELISA）. Pathological changes in livers were observed by HE staining. Result： Compared with the model group, the Danggui Shaoyao San group showed significant improvement in live indexes, with notable decrease in serum ALT and AST and the time of PT, improvement in liver pathological changes. Simultaneously, the amount of ascites decreased to varying degrees, with notable increase in urine in 24 h and decrease in AVP concentration in plasma. Conclusion： Danggui Shaoyao San can notably improve liver functions of rats with cirrhotic ascites, reduce the generation of ascites and delay the progress of liver pathological changes. Its mechanism may be related to AVP.