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毛细管电泳法测定石榴皮中鞣花酸与安石榴苷含量 被引量:8

Determination of Ellagic Acid and Punicalagin in Pomegranate Peel by Capillary Electrophoresis
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摘要 建立了毛细管电泳紫外检测法测定石榴皮中鞣花酸和安石榴苷含量的方法,并对不同产地石榴中的石榴皮鞣花酸和安石榴苷含量进行测定。研究了运行缓冲液pH值和浓度、运行电压等对电泳的影响,得到最优化的测定条件为:50 mmol/L的硼砂-硼酸缓冲液(pH 8.7),分离电压20 kV,电动进样9 s/20 kV。在最优条件下,检测波长260 nm,上述两种组分可在12 min内完全分离。结果显示,鞣花酸和安石榴苷的线性范围分别为5.0×10-7~5.0×10-5g/mL和2.0×10-6~5.0×10-5g/mL,检出限分别为1.0×10-7g/mL和1.3×10-6g/mL,峰面积的相对标准偏差(RSD,n=7)分别为2.4%和2.2%,平均回收率分别为101%和98%。采用高效液相色谱法进行比对,t-检验表明两组数据无显著性差异。该法简便、快速、准确,已成功应用于实际样品的测定。 A capillary electrophoresis(CE) with UV detection method was developed for the determi- nation of ellagic acid and punicalagin in pomegranate peel, grown in different origins. Effects of some important factors, such as pH value, concentration of running buffer, separation voltage and injection time were investigated. The optimum conditions were as the follows: running buffer: 50 mmol/L borax buffer(pH 8.7), separation voltage: 20 kV, injection time: 9 s/20 kV. Under the optimum conditions, two analytes could be separated within 12 min at the detection wavelength of 260 nm. The good linearities between peak area and mass concentration were obtained in the ranges of 5.0×10^-7 - 5.0×10^-5 g/mL and 2.0×10^-6-5.0× 10^-5 g/mL for ellagic acid and punicalagin, with their detection limits of 1.0×10^-7g/mL and 1.3×10^-6g/mL, respectively. The relative stand- ard deviations ( RSD, n = 7 ) for the peak areas of ellagic acid and punicalagin were 2.4% and 2.2% , respectively. The average recoveries were 101% for ellagic acid and 98% for puniealagin It is found by t-test that there were no significant differences between this method and the HPLC method. The proposed method was simple, rapid and accurate, and was successfully applied in the determination of the analytes in pomegranate peel samples.
出处 《分析测试学报》 CAS CSCD 北大核心 2013年第3期367-371,共5页 Journal of Instrumental Analysis
关键词 毛细管电泳 石榴皮 鞣花酸 安石榴苷 capillary electrophoresis pomegranate peel ellagic acid punicalagin
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