盐胁迫下红砂愈伤组织的抗氧化能力与耐盐性研究

Study on the Antioxidative Ability and Salt Tolerance of Reaumuria soongorica Callus under Salt Stress

通过测定不同梯度盐胁迫下红砂愈伤组织中超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)的活性,以及丙二醛(MDA)含量和细胞膜透性,探讨了红砂抗氧化能力与耐盐性的关系。结果显示:随着盐胁迫浓度增加,红砂愈伤组织中MDA含量比对照增加0.84%～51.03%,SOD、POD和CAT活性分别比对照提高9.83%～51.98%、26.4%～140.2%和44.74%～216.34%。表明红砂可以通过上调SOD、POD和CAT等保护酶的活性来响应盐胁迫,并以此降低盐胁迫诱导的膜脂过氧化程度,从而提高其耐盐性。

The mechanism of the enduribility of Reaumuria soongorica to habitat with salt stress was investigated by measuring the activities of superoxide dismutase （SOD）, peroxidase （POD） and catalase （CAT）, the content of malondialdehyde （MDA）, and the permeability of cell membrane of the callus in vitro. The results showed that, with progressing salt stress, the activities of SOD, POD and CAT were elevated significantly by 9.83%--51.98%, 26.4%--140. 2% and 44.74%--216.34%, respectively, in comparison to the control treatment, the content of MDA increased by 0.84%- 51.03%. It suggested that R. soongorica could adapt well to the habitat with salt stress by reducing the accumulation of reactive oxygen species （ROS） and the subsequent acceleration of lipid peroxide induced by excessive salt.