关中奶山羊隐性乳房炎病原菌的分离鉴定

Islolation and Identification of Pathogenic Bacteria in Guanzhong Dairy Goats with Subclinical Mastitis

为摸清近年来陕西关中奶山羊隐性乳房炎病原菌种类,利用奶山羊隐性乳房炎诊断试剂盒对陕西富平关中奶山羊进行随机抽样检测,采集乳房炎阳性乳样进行细菌的分离与鉴定。结果显示,从364份阳性样品中共分离出337株细菌,其中凝固酶阴性葡萄球菌186株,占所分菌的55.2%;金黄色葡萄球菌50株,占所分菌的14.8%;乳房链球菌24株,占所分细菌的7.1%;猪链球菌9株,占所分细菌的2.7%;大肠埃希菌61株,占所分细菌的18.1%;其他细菌7株,占所分细菌的2.1%。表明凝固酶阴性葡萄球菌是引起奶山羊隐性乳房炎的主要病原菌,其次是大肠埃希菌,金黄色葡萄球菌,链球菌。本研究结果为奶山羊乳房炎防治和鲜乳质量控制提供了重要的依据。

In order to clarify the kind of pathogens causing mastitis in Guanzhong dairy goats in recent years, dairy goats were detected randomly by the kit of subclinical mastiffs detection in Fuping of Shaanxi Province, and the bacteria were isolated and identified from the positive milk samples. 337 strains were i- dentified from 364 samples. 186 strains of coagulase-negative staphylococci （55.2%）, 50 strains of Staph- ylococcus aureus （ 14. 8%） , 24 strains of Streptococcus uberis （7. 1 %）, 9 strains of Streptococcus suis （2.7%）, 61 strains of Escherichia coli （18. 1%） and 7 strains of other bacteria （2. 1%） were isolated. The results showed that coagulase-negative staphylococci were the predominant pathogens in Guanzhong goats with subclinical mastitis and Staphylococcus aureus, Escherichia coli, Streptococcus were also im- portant. The report plaid a reference for preventing mastitis and controlling raw milk quality in dairy goats.