实验性2型糖尿病大鼠模型的建立

A NOVEL RAT MODEL OF TYPE 2 DIABETES MELLITUS

目的 :制备一种发病过程类似人类 2型糖尿病的动物模型。 方法 :雌性Wistar大鼠喂以高糖高脂饲料 (其中含 10 %蔗糖 ,10 %猪油 ,5 %胆固醇 )一个月 ,诱发出胰岛素抵抗 ,继以低剂量链菌素 (STZ ,2 5mg/kg ,腹腔注射 ) ,诱发胰岛素代偿性分泌障碍 ,使之产生高血糖症。 结果 :高糖高脂饮食后一个月 ,大鼠出现高胰岛素血症和胰岛素抵抗 ,高血压 ,高脂血症。STZ注射后 ,大鼠血糖升高 (15 5± 7 80mmol/L ,常规饲料喂养大鼠为 5 70±0 90mmol/L ,P <0 0 5 ) ,血胰岛素降至常规饲料喂养大鼠水平 ,其它特征继续存在。 结论 :本研究通过饮食方法成功复制出了 2型糖尿病模型 ,它具有中度高血糖 ,高血脂 ,高血压 ,血胰岛素不低 ,胰岛素抵抗 ,成功率高等特点 ,是 2型糖尿病血管并发症及其药物研究的理想动物模型。

The study was aimed at developing a rat model that closely simulates the metabolic abnormalities of human type 2 diabetes mellitus. METHODOLOGY Female Wistar rat was fed with a diet enriched with sucrose (10%,w/w),triglyceride(10%,w/w)and cholesterol(5%,w/w)to induce insulin resistance,and after one month on the diet streptozotion(STZ,25 mg/kg)was injected intraperitoneally to induce hyperglycemia. RESULTS Insulin levels increased in response to the diet(33 5±1 97 mU/L vs 18 3±2 04 mU/L in controls, P <0 01)and insulin resistance was confirmed by insulin suppression test.After STZ injection hyperglycemia(15 5±7 80 mmol/L vs 5 70±0 90 mmol/L in controls, P <0 05)was observed,and plasma insulin decreased to levels of chow-fed rats,with hypertension and hypertriglyceridemia and hypercholesterolemia. CONCLUSION This novel rat model simulates human type 2 diabetes in moderate hyperglycemia,hypertension,dyslipidemia and insulin resistance.It may provide a particular advantage for investigating diabetic vascular complications and pharmacology.