QuEChERS法结合GC-NPD测定果蔗蔗汁中有机磷农药残留量

Determination of Organphosphorus Pesticides in Chewing Cane Juices by QuEChERS Cleanup and GC-NPD

[目的]建立果蔗中有机磷农药残留量的快速检测方法,为果蔗安全食用提供保障。[方法]运用QuEChERS法结合GC-NPD建立了果蔗中6种有机磷农药(敌敌畏、甲基毒死蜱、马拉硫磷、倍硫磷、毒死蜱、三唑磷)残留量的快速分析方法。[结果]QuEChERS法明显优于传统提取法,可有效提取果蔗样品中6种有机磷农药,大大节约了使用试剂和提取时间。6种有机磷农药均呈良好的线性关系;在0.020～0.100 mg/L的添加水平,6种有机磷农药的平均加标回收率为68.58%～92.85%,相对标准偏差在2.16%～9.89%,方法的最低检出限为0.006 8～0.012 0 mg/L。[结论]QuEChERS法的灵敏度、准确度和精密度均符合农药残留测定的技术要求,不仅能有效缩短样品处理周期,还能保证样品峰无杂质干扰,回收率也符合残留分析的要求。

[Objective] To establish a method for determination of organphosphorus pesticides in chewing cane juices and prodivide safety guaranteeing.[Method] By using QuEChERS and GC-NPD method,a rapid analysis method for six kinds of organphosphorus pesticides residue in sugarcane juices was established,which includes dichlorvos,chlorpyrifos-methyl,malathion,fenthion,chlorpyrifos and triazophos.[Result]QuEChERS method could extract six organphosphorus pesticides residue in chewing cane juices effectively,which saved the organic reagent and time.Good linearity was found in different standard curves,the detection limits of apparatus was 0.020-0.100 mg/ml,recovery rate was 68.58%-92.85%,and relative standard deviation（RSD） was in the range of 2.16%-9.89%.[Conclusion] The sensitivity,accuracy and precision of QuEChERS method were consistent with technical requirements of pesticide residue determination.It not only can shorten sample processing cycle effectively,but also ensure that no impurity was in the sample peak and the recovery rate is also consistent with the requirements of residual analysis.