火针治疗类风湿关节炎大鼠疗效及安全性评价

Efficacy and safety evaluation of fire needling for rats with rheumatoid arthritis

目的:探讨火针对类风湿关节炎大鼠的治疗作用和安全性。方法:将40只雌性Wistar大鼠随机分为正常组、模型组、甲氨蝶呤(MTX)组、火针组,每组10只。正常组大鼠右后足跖底部皮下注射0.1mL 0.9%氯化钠溶液,其余各组于右后足跖底部皮下注射0.1mL完全弗氏佐剂进行造模。火针组点刺"夹脊"穴、"足三里"和"阿是穴",深度5mm,3日治疗1次,共8次;MTX组每只大鼠按灌胃量2.0mg/kg,每7天给药1次,共4次。观察各组大鼠体质量、足容积肿胀度、关节疼痛评分、多关节炎指数变化;光镜观察大鼠肝脏组织的病理变化。结果:模型组大鼠体质量、足容积肿胀度、关节疼痛评分、多关节炎指数较正常组显著升高(P<0.05,P<0.01),治疗后上述各指标两治疗组较模型组均明显降低(均P<0.01),并且火针组与MTX组相比差异均有统计学意义(P<0.05,P<0.01)。与正常组比较,模型组大鼠肝细胞之间出现呈簇集状分布的炎性细胞,MTX组和火针组肝细胞之间炎性细胞减少,MTX组肝细胞变性水肿、肝细胞核增大变形。结论:火针治疗佐剂性关节炎(AA)大鼠疗效显著,对肝脏无损伤,能更好地控制类风湿关节炎的病情发展。

Objective To explore the therapeutic effect and safety of fire needling on rat with rheumatoid arthritis. Methods Forty female Wistar rats were randomly divided into a normal group, a model group, a methotrexate （MTX） group and a fire needling group, 10 rats in each group. The 0.1 mL of normal saline was subcuta neously injected in rats right back paw in the normal group, while the other groups received subcutaneous injection of 0.1 mL of complete Freund＇s adjuvant to establish the model. The pricking method was applied in the fire needling group at ＂Jiaji＂ （EXB 2）, ＂Zusanli＂ （ST 36） and Ashi points with a depth of 5 ram, once every three days, and totally eight times were required. In the MTX group, with intragastric administration of 2.0 mg/kg per rat, the treatment was given once every seven days, and totally four times were required. The weight, swelling rate of foot, joint pain score and polyarthritis index of rats in each group as well as the pathological change of liver tissue under light microscope were observed. Results The weight, swelling rate of foot, joint pain score and pol yarthritis index in the model group were significantly higher than those in the normal group （P〈0.05 ,P〈0.01）, after the treatment, the abovementioned indexes in the two treatment groups were obviouly dereased as componed with those in the model group （all P〈0.01）, and there were statistical differences between the fire needling group and MTX group （P〈0.05 ,P〈0.01）. Compared with the normal group, inflammatory cells were appeared with a cluster distribution in the liver cells of rats in the model group. The inflammatory cells were reduced in the MTX group and fire needling group, and liver cells had degenerative edema and cell nucleus were largen and deformed in the MTX group. Conclusion The fire needling has significant efficacy for rats with adjuvant arthritis （AA） wi thout any damage to the liver, which coud have a better control of disease progression of rheumatoid arthritis .