5-氮杂-2'-脱氧胞苷对肺癌细胞系BE1和LTEP-α-2中Axin基因表达及细胞增殖的影响

The effect of 5-Aza-2'-deoxycytidine on Axin expression and proliferation in lung cancer cell line BE1 and LTEP-α-2

目的本研究的目的是探讨5-氮杂-2'-脱氧胞苷(5-Aza-2'-deoxycytidine,5-Aza)对人肺癌细胞系BE1及LTEP-α-2的增殖和侵袭的调节及其可能的机制。方法两个细胞系均应用5-Aza进行处理,并应用MTT法检测两细胞系经5-Aza处理后细胞增殖能力的变化,Transwell法检测了两细胞系处理后侵袭能力的变化,Westernblot检测Axin、β-catenin、cyclin D1和MMP-7的表达。结果 5-Aza抑制BE1和LTEP-α-2细胞的增殖和侵袭能力(<0.05),与LTEP-α-2细胞系相比,BE1细胞系受抑制程度更为明显(<0.05)。5-Aza上调BE1细胞中Axin的表达,抑制β-catenin、cyclin D1和MMP-7的表达,但对LTEP-α-2细胞中Axin等的表达没有明显的影响。结论 5-Aza可以抑制肺癌细胞的增殖和侵袭,BE1细胞中的Axin基因可能存在异常高甲基化,高甲基化的Axin基因可能成为未来治疗肺癌的靶点。

Objective To explore the effect of 5-aza-2＇-deoxycytidine（5-Aza） treatment on the proliferation and inhibition of human lung cancer cell line BE1 and LTEP- a -2 and their possible mechanism. Methods Two cell lines were treated with 5-Aza, MTr assay was used to test the proliferation of the two cell lines with or without 5-Aza, transwell was used to examine the invasion of the two cell lines with or without 5-Aza. Western blot was used to detect the expression of Axin, 13-catenin, cyclin D1 and MMP-7 in the two cell lines with or without 5-Aza. Results 5-Aza could inhibit the proliferation and invasion of BE1 and LTEP- ct -2 cells （P〈0.05）, compared with LTEP- ct -2 cell line, BE1 cell lines were inhibited more significantly after 5-Aza（p〈0.05）. 5-Aza could upregulate the expression of Axin and inhibit the expressions of 13-catenin, cyclin D1 and MMP-7 in BE1 cells but not in LTEP-ct-2 cells. Conclusion 5-Aza could inhibit the proliferation and invasion of lung cancer cells, abnormal hypermethylated Axin gene might be detected in BE1 cells, hypermethylated Axin gene might become a target for clinical lung cancer treatment.