摘要
目的:研究京尼平苷(Geniposide)对硝普钠(SNP)诱导软骨细胞凋亡与细胞周期的影响。方法:3周龄SD大鼠,用于分离培养软骨细胞。将第Ⅱ代软骨细胞进行分组,噻唑兰染色(MTT)法检测细胞增殖,流式细胞仪检测细胞周期及凋亡率,硝酸还原酶法检测培养液中NO含量。结果:京尼平苷干预SNP诱导软骨细胞凋亡后,细胞处于G0/G1期的百分比减少,S及G2/M期的细胞百分比增加;同时显著降低软骨细胞凋亡率及培养液中NO含量(r=0.917,P<0.01)。结论:京尼平苷干预SNP诱导软骨细胞凋亡,可降低培养液中NO含量,促进细胞增殖,这可能是京尼平苷治疗骨性关节炎的作用机制之一。
Objective : To study the effects of Geniposide on SNP (sodium nitroprusside)-induced apoptosis of chondrocyte in vitro and cell cycle. Methods:The chondrocyte of three-week-old SD rats were separated and cultivated. The second genera- tion of chondrocyte cells were involved in experiment. Cbondrocyte proliferation was measured by MTlr assay;flow cytometer were adopted to observe cell cycle and apoptosis rate;NO examination adopted nitrate reductase method. Restflts:Geniposide could significantly decrease the percentage of SNP-induced chondrocytes in G0/G1 phase and increased percentage in S phase and G2/M phase. The apoptosis of chondrocyte and the concentration of NO in the culture supernatants was reduced signifi- cantly (r=0.917, P〈0.01 ). Conclusion: Geniposide could impact SNP-induced apoptosis of chondrocyte by reducing the con- centration of NO in the culture supernatants, promoting proliferation of chondrocytes, which is a probable and important mech- anism of Geniposide preventing osteoarthritis.
出处
《中国骨伤》
CAS
2013年第3期232-235,共4页
China Journal of Orthopaedics and Traumatology
基金
湖北省自然科学基金资助项目(编号:2010CDB10701)~~