中药材二氧化硫残留快速检测方法的建立

Establishment of Rapid Detection Method for Sulfur Dioxide Residues in Chinese Herbal Medicines

目的:建立硫熏中药材中二氧化硫残留的快速检测方法。方法:采用分光测色计进行颜色测定,利用亚硫酸钠与邻苯二甲醛、乙酸铵溶液在一定条件下反应生成带有紫色物质的原理,对20批中药材进行检测,并半定量其二氧化硫残留量。结果:亚硫酸钠加入量与溶液颜色色度在0～25.23μg线性关系良好,与标准加入量比对,可确定样品的二氧化硫含量范围。结论:该法简便、快速,灵敏度较高,可为中药材二氧化硫的快速检测及其半定量提供参考。

Objective：To establish a fast detection method of sulfur dioxide residues in stoved traditional Chinese medicine（TCM）.Method：Colour chromaticity was detected by spectrocolorimeter,taking advantage of the principle of purple substance creation by sodium sulfite reacting with OPA and ammonium acetate under certain conditions,20 batches of TCM were detected,and their sulfur dioxide residues were semi-quantitative.Result：A linear relationship was obtained between color chromaticity and the addition of sodium sulfite in the range of 0-25.23 μg,the range of sulfur dioxide residues in samples could be determined by comparing with the standard addition of sodium sulfite.Conclusion：This method was simple,rapid and sensitive,it could provide a reference for fast detection and half quantification of sulfur dioxide residues in TCM.