虎耳草乙酸乙酯部位高效液相色谱指纹图谱研究

HPLC fingerprint of ethyl acetate extraction of Saxifraga stolonifera

目的:建立虎耳草药材的乙酸乙酯部位HPLC指纹图谱分析方法。方法:以Diamonsil C18色谱柱(4.6mm×250mm,5μm),流动相乙腈(A)-0.05%磷酸水溶液(B),梯度洗脱,流速1.0mL·min-1,检测波长256nm,柱温30℃。结果:建立了虎耳草药材的乙酸乙酯部位指纹图谱,确定了15个共有峰,并用对照品指认了7个峰,精密度和重复性实验中各共有峰相对保留时间RSD均小于3%,11批不同产地虎耳草相似度存在差异。结论:虎耳草药材乙酸乙酯部位指纹图谱的建立为药材质量控制提供了科学依据。

Objective： To establish an HPLC fingerprint of ethyl acetate extraction of Saxifraga stolonifera. Method： The HPLC analysis was performed on a Diamonsil Cls （ 4. 6 mm × 250 mm, 5 μm）column with isocratic elution of acetonitrile-0. 05% phosphoric acid at a flow rate of 1.0 mL· min-1. The detection wavelength was set at 256 nm and the column temperature was set at 30 ℃. Result： The HPLC fingerprint of ethyl acetate extraction of S. stolonifera has been established. There were fifteen common peaks, seven of which were identified by reference substances. The RSD of relative retention time was less than 3% in the precision and repeated tests. Eleven samples from different area can be distinguished from their fingerprints. Conclusion： This method is reasonable and reliable and can be used for quality control of S. stolonifera.