14种中草药对肠源性益生菌及出血性大肠杆菌的抑菌作用

Inhibitory effects of 14 kinds of Chinese herbal medicine against enterogenic probiotics and enterohemorrhage E. coli

采用水煎法从石菖蒲等14种中药中提取有效成分,琼脂板扩散法观察有效成分对4株肠源性益生菌及1株出血性大肠杆菌抑菌效果。结果表明:川芎、黄芩、板蓝根、大黄、甘草对4株肠源性益生菌抑制作用较强,抑菌圈直径最高可达22mm,葛根、陈皮、穿心莲、苍术对4株肠源性益生菌表现的抑制作用相对较弱,抑菌圈直径最高为11mm,其他中药对4株益生菌无抑制作用;黄芩、黄芪、石菖蒲、陈皮、甘草、苍术对出血性大肠杆菌表现出抑菌作用,抑菌直径为10～24mm,其中黄芩抑菌作用最明显,且黄芪提取物对出血性大肠杆菌的MIC为0.625g/mL,均小于4种益生菌,川芎、葛根、丹皮、板蓝根、知母、穿心莲、大黄、茯苓则未表现出抑菌性。

Active ingredients of 14 kinds of Chinese herbal medicine were extracted using decoc- tion method, and their inhibitory effects on four enterogenic probiotics strains and one entero- hemorrhage E. coli （EHEC） strain were tested with disc diffusion method, respectively. The results showed that Ligusticum wallichii Franch, Scutellaria baicalensis Georl, Isatidis Ra- dix, Rheum officinale Baill and Glycyrrhiza uralensis Fisch had high antibacterial effects on the four enterogenic probiotics strains, and the bacteriostatic diameter was 22 ram; Pwraria lobata （Willd） Ohwi, Pericarpium Cirri Reticulatae , Andrographis paniculata （Burm. f. ） Ness and Atractylodes chinensis （DC.） Koidz. had lower antibacterial abilities, the bacterio- static diameter was 11 ram,and the others had no antibacterial ability; Scutellaria baicalensis Georgl, Astragalus membranaceus （Fisch.） Bunge, Acorus gramineus Soland and Pericarpium Cirri Reticulatee , Glycyrrhiza uralensis Fisch. Atractylodes chinensis（DC. ）Koidz had antibacterial effects on EHEC strain, the bacteriostatic diameter was 10-- 24 mm, Scutellaria baicalensis Georgl had the biggest significant effect of inhibition on EHEC. MIC of Astragalus membrana- ceus（Fish. ）Bunge to EHEC were 0. 625 g/mL , less than the four kinds of probiotics,Ligusti- cure wallichii Franch, Pueraria lobata （Willd.）Ohwi, Paeonia suf fruticosa Andr, Isatidis Radix L. , Anemarrhena asphodeloides Bunge, Andrographis paniculata （Burm. f. ） Nees, Rheum officinale Baill and Poria cocos（Schw. ） Wolf had no inhibition on EHEC strain.