摘要
以MTT法观察补肾和柔肝中药含药血清对体外软骨细胞的影响。结果 :在两次连续灌胃 3h后所取血清浓度为 5 %和 10 %时 ,对软骨细胞有促进增殖的作用 ;血清浓度为 5 %时 ,两药之间作用无差异 ,而 10 %时 ,补肾方作用优于柔肝方。为便于以下比较 ,将血清浓度定在 5 % ;长时间 ( 8d)灌胃和短时间 ( 1d)所取血清对软骨细胞的增殖作用无差异 ;以 0 5、1、5倍等效剂量灌胃所取血清对软骨细胞的增殖作用未呈量效依赖关系 。
MTT method was used to observe influence of drug containing serum in the herbal drugs to tonify the kidney and soften the liver on extrinsic chondrocyte.The results showed when serum concentration collected 3h after continuous stomach feeding twice was 5% and 10%,there was a promoting function for proliferation of chondrocyte,when serum concentration was 5%,there was no difference between two groups of the herbal drugs.While in 10%,the kidney tonifying formula was better than the liver softening formula.For convenience of the following observation,serum concentration was decided in 5%.There was no difference in the proliferating function on chondrocyte in the serum collected after long term(8d)and short time(1d)stomach feeding.The proliferating function of the serum collected after stomach feeding with 0 5,I and 5 times of the equivalent dose on chondrocyte did not present the dose effect dependent relationship,with the best effect in the equivalent dose.
出处
《上海中医药大学学报》
CAS
2000年第1期43-45,共3页
Academic Journal of Shanghai University of Traditional Chinese Medicine
基金
国家自然科学基金资助项目!(39870934)
关键词
软骨细胞
MTT法
中药
含药血清
Chondrocyte
MTT method
Chinese herbal medicine
drug containing serum.