兔骨髓干细胞和脂肪干细胞增殖和分化能力的比较

Comparison of Proliferation and Differentiation Capacity of Rabbits' Mesenchymal Stem Cells Derived from Bone Marrow and Adipose Tissue

目的:评价两种不同来源的间充质干细胞,即骨髓干细胞(BMSC)和脂肪干细胞(ADSC)增殖和分化能力,为其将来更好的应用于细胞治疗和组织工程提供一定的理论支持和依据。方法:分别取同一只兔的髂骨骨髓和其腹股沟脂肪作为BMSC和ADSC的来源,分离培养后比较其生物学特性包括细胞的增殖能力(MTT),成骨成脂分化潜能,细胞表面抗原标记物。结果:BMSC类似纤维细胞样,呈长梭形紧密排列;而ADSC细胞整体呈旋涡状排列;但ADSC倍增时间更短,增殖速度更快;两者都具有向成骨成脂分化的潜能,但BMSC的成骨能力更好。结论:ADSC有更短地倍增时间和更快地增殖速度;两者都具有向成骨成脂分化的潜能,但相较于ADSC更强的成脂分化能力,BMSC的成骨分化能力更好。两者都可以作为组织工程的种子细胞。

Objective： To evaluate the proliferation and differentiation capacity of rabbits＇ mesenchymal stem cells derived from bon~ marrow （BMSC） and adipose tissue （ADSC）, which aimed to provide a theoretical support for cell therapy and tissue engineering applications in the future. Methods： BMSC and ADSC were obtained from the same rabbit＇s iliac bone marrow and inguinal fat respectively; we compared the biological characteristics of the cells including their proliferation ability （MTT）, multi-differentiation potential, cell surface antigen markers after the cell isolated and cultured. ReSults： When BMSC presented fibroblast-like and fusiform closely spaced growth, ADSC presented fibroblast-like cells with the spiral-shaped, radial arranged; the doubling time of ADSC is much shorter, and the proliferation speed is much faster; they both have multilineage differentiation potentials. BMSC have the better osteogenic capacity. Conelusion： ADSC have a much shorter doubling time and a much faster proliferation speed; but compared to the ADSC more adipogenic differentiation capacity, BMSC osteogenic differentiation capacity is much better. Both of them can be used as seed cells for tissue engineering.