茸菖胶囊对戊四唑致痫大鼠学习记忆能力及海马苔藓纤维出芽的影响

Effect of Rongchang capsule on learning memory and hippocampal mossy fiber sprouting in rats with epilepsy induced by pentylenetetrazole

[目的]观察茸菖胶囊对戊四唑致癫痫大鼠学习记忆能力及海马组织苔藓纤维发芽(MFS)的影响。[方法]以戊四唑点燃大鼠为模型,随机分为中药高剂量组、中药中剂量组、中药低剂量组、丙戊酸钠组、模型组、正常组,水迷宫实验记录逃避潜伏期和跨越平台次数,戊四唑惊厥阈实验测定惊厥潜伏期,Timm染色法观察海马苔鲜纤维出芽。[结果]各治疗组大鼠与治疗前相比,均可减少大鼠的发作程度,水迷宫实验:中药组逃避潜伏期和跨越平台次数均明显短于模型组(P>0.05)。Timm染色结果表明:各治疗组海马CA3区及齿状回分子层Timm染色颗粒MFS评分低于模型组(P<0.01)。[结论]茸菖胶囊可以有效控制癫痫大鼠的发作次数及级别,改善戊四唑致癫痫大鼠的学习记忆能力,其作用机制与抑制海葬纤维异常出芽,从而保护海马神经元有关。

[Objective] To explore the effect and mechanism of Rongchang capsule （RCC capsule） on learning and memory and the mossy fiber sprouting （MFS）of hippocampus in rats with epilepsy. [Methods] The 48 rats were randomly divided into normal group, model group, valproic acid sodium （VPA） group and RCC Capsule low, middle, high dosage group. To establish the model of epileptic rats kindled by PTZ. All test rats were treated for 28 days. The water maze test was assigned to evaluate RCC capsule effects on learning and memory capacity of rats. By Timm vulcanization silver staining method to observe hippocampal moss fiber budding and hippocampal structure damage were observed. [Results] Every treated group can reduce the rat attack degree water maze experiment results of RCC groups showed escape latency and number of cross platform in each group were shorter than that of the model group （P〈0.05）. Timm dying results show that the total area of MFS of every treatment group in CA3 hippocampus beginning layer and the dentate gyrus Timm dyeing particles molecular layer less than the model group （P〈0.01）. [Conclusion] RCC capsule can effectively control the attack frequency and level of the epilepsy, and improve the ability of learning and memory of epilepsy rats. It might inhibit MFS, then protect the hippocampal neurons to improve learning and memory capacity.