摘要
目的探讨黄芪注射液对小鼠非酒精性脂肪肝的保护作用。方法将健康雄性小鼠36只随机分为正常对照组、非酒精性脂肪肝模型组和黄芪注射液给药组[黄芪注射液20 mg/(kg.d)灌胃],除正常对照组用普通饲料喂养外,其余各组均给予高脂饲料喂养并每周皮下注射四氯化碳1次。6周后处死小鼠,收集血清和肝组织,检测血清丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)活性以及血清和肝组织中三酰甘油(TG)、总胆固醇(TC)含量,同时进行肝组织HE染色检测肝脏病理改变。结果与正常对照组比较,模型组小鼠血清ALT、AST、TG、TC及肝组织TG、TC明显升高,其肝脏病理检查示脂肪变性,呈中、重度炎症变化。黄芪注射液给药组小鼠血清ALT、AST、TG、TC和肝组织TG、TC降低,且肝脂肪变性和炎症坏死程度减轻。结论黄芪注射液对小鼠非酒精性脂肪肝具有良好的干预作用。
Objective To study the protective effect of Huangqi Injection on nonalcoholic fatty liver disease(NAFLD) in mice.Methods 36 healthy male mice were randomly divided into three groups:the normal control group,the nonalcoholic fatty liver model group and the Huangqi Injection treatment group.The normal control group was fed with normal diet,while the other groups were fed with high-fat diet and given by hypodermic injection of CCl4 once per week.The Huangqi Injection treatment group was administered by intragastrical injection with Huangqi Injection 20 mg/(kg·d).After 6 weeks,all the mice were sacrificed for collecting serum and liver tissues.The serum alanine aminotransferase(ALT),aspartate aminotransferase(AST),triglycerides(TG),total cholesterol(TC) and the hepatic TG,TC were measured.The pathological changes in mice liver were observed by HE staining.Results Compared with the normal control group,the serum ALT,AST,TG,TC and the hepatic TG,TC in the model group were markedly increased.The model group presented with steatosis,and moderate or severe inflammation by the liver pathological examination.Compared with the model group,the serum ALT,AST,TG,TC,and the hepatic TG,TC in the Huangqi Injection treatment group were decreased,while the liver adipose degeneration and the degrees of inflammation and necrosis were alleviated.Conclusion Huangqi Injection exerts better intervention effect on NAFLD in mice.
出处
《现代医药卫生》
2013年第9期1297-1298,共2页
Journal of Modern Medicine & Health
关键词
黄芪
注射剂
脂肪肝
中药疗法
小鼠
非酒精性脂肪肝
Astragalus membranaceus
Injectio
Fatty liver/zhong yao liao fa
Mice
Nonalcoholic fatty liver disease