盐诱导纳米金基因探针杂交及其应用研究

Salt-induced Hybridization and Its Clinical Application Based on Nano Gold Probe

因为DNA双螺旋结构的稳定性对周围环境介质非常敏感,通过分析不同盐浓度缓冲溶液中纳米金基因探针的杂交效率,发现由于纳米金对于基因探针的末端修饰作用,使得错配DNA链的双螺旋结构更加不稳定,尤其在盐浓度较低的缓冲体系中,不稳定性差异更为明显。通过优选出特定盐浓度值,以稳定性的明显差异来区分完全配对和错配基因序列,通过纳米金探针紫外-可见光谱吸光强度,定量检测分析目标DNA,结果显示,这是一种非常简单经济且实用的检测单个错配基因序列的新方法。

This study was dependent on the stability of the duplex DNA structure in different surrounding media,and therefore the effects of the salt concentration in the hybridization buffer on DNA hybridization were thoroughly investigated.Due to the effect of the gold nanoparticles modification,we found the stability of single-mismatched DNA（MMT） structure is very sensitive to the salt concentration of the hybridization buffer,especially,the lower of the salt concentration,the more remarkably difference of the stability.Based on it,the optimized salt concentration was used as a stringency tool to discriminate single-mismatched DNA（MMT） from perfectly matched DNA（PMT）.Therefore,quantitative information concerning the target analyte could be quantitatively translated by measuring the absorbance of gold nanoprobes.The results indicated this to be a very simple and economic strategy for detection of single-mismatched DNA strands.