摘要
目的建立经口灌胃途经给予造模致敏原的挪威棕色(Brown Norway,BN)大鼠致敏动物模型。方法选用不同周龄(4周龄和8周龄)及不同性别(雄性和雌性)的BN大鼠,每天经口灌胃给予不同剂量(10.0、1.0、0.1mg)的造模致敏原—卵清蛋白(OVA),共35天。在第28和35天分别进行内眦静脉取血并分离血清,采用酶联免疫吸附测定(ELISA)方法检测血清中OVA特异性IgE(OVA sIgE)浓度。结果中剂量组雌性4周龄和8周龄BN大鼠血清中OVA sIgE浓度在第28和35天较阴性对照组差异有统计学意义(P<0.05);低剂量组雄性8周龄BN大鼠血清中OVA sIgE浓度在第28天较阴性对照组差异有统计学意义(P<0.05);各组雄性4周龄BN大鼠血清中OVA sIgE浓度在第28和35天均较阴性对照组差异无统计学意义。结论经口灌胃途径给予不同性别和周龄的BN大鼠不同剂量的OVA,雌性大鼠比雄性大鼠更敏感;周龄对敏感性无影响;1.0 mg的致敏剂量较适合。因此,选用雌性BN大鼠,每天经口灌胃给予1.0 mg OVA,28~35天即可建立比较理想的BN大鼠经口致敏动物模型。
Objective To establish an oral Brown Norway (BN) rat model for food allergy. Methods Different doses (0. 1, 1. 0, and 10.0 rag/d) of ovalbumin (OVA) were administered to male and female BN rats with different age (4 and 8 weeks) by gavage for 35 days. Specific serum IgE against OVA on the 28th and 35th days was analysed by enzyme- linked immunosorbent assay (ELISA). Results Daily gavage of 1.0 mg OVA to 4-week female and 8-week female BN rats resulted in significantly higher concentrations of OVA sIgE on day 28 and 35 compared to the control group (P 〈0. 05). On day 28, the concentration of OVA slgE was significantly higher in 8-week male BN rats exposed to 0. 1 mg OVA/day than the control group (P 〈 0. 05). The concentration of OVA slgE had no significant difference among all groups of 4-week male BN rats on day 28 and 35. Conclusion Different doses of OVA were administered orally to BN rats with different sex and age. Females were more sensitive than males; age did not significantly influence the concentration of OVA slgE; the better dose was 1.0 mg. Therefore, an ideal animal model of food allergenicity can be established by 1.0 mg/d OVA garage to female BN rats for 28 - 35 days.
出处
《中国食品卫生杂志》
北大核心
2013年第3期214-217,共4页
Chinese Journal of Food Hygiene
基金
转基因重大专项(2011ZX08011-005)