食管癌中3p24和11p15.5染色体位点潜在抑癌基因的研究

Putative tumor suppressor genes on chromosome 3p24 and 11p15.5 in esophageal carcinoma

目的 :探讨 3p2 4染色体位点的杂和缺失和 11p15 .5位点的点突变同食管癌之间的相关性 ,寻找这两个位点中潜在的抑癌基因 ,并希望通过此研究能为最后确定该基因的功能奠定基础。 方法 :用 PCR- RFL P法检测3p2 4染色体的三个位点 (EAβ MD、EAβ H、EAβ R)上等位基因的杂合缺失情况 ,PCR- SSCP法检测 11p15 .5位点的点突变。 结果 :经 RFL P法显示 :在 3p2 4的 EAβ MD位点 ,杂合缺失率为 75 % ,EAβ H位点的杂合缺失率为5 0 % ,EAβ R位点的杂合缺失率为 6 .2 5 % ;SSCP法未发现 11p15 .5位点存在点突变。 结论 :EAβ MD和 EAβ H位点的杂合缺失率较高 ,此两位点可能存在抑癌基因 。

Objective: To investigate the role of these chromosome 3p24 deletions and chromosome 11p15.5 mutations in the pathogenesis of esophageal carcinomas. Methods: DNA was extracted from fresh tumours. Loss of heterozygosity was assessed by PCR RFLP and chromosome mutations was detected by PCR-SSCP. Results: On the EAβMD site of 3p24, the LOH was detected 9 of 12(75%), EAβH site was 5 of 10(50%) and EAβR site was 1of 16(6.25%) respectively. No mutation on chromosome 11p15.5 was detected. Conclusion: The results suggest that inactivation of the putative tumor suppressor genes on chromosome 3p site EAβMD and site EAβH play important roles in the development of esophageal carcinoma.