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培养条件对荞麦愈伤组织生长及黄酮合成的影响 被引量:15

Influence of Culture Condition on Callus Growth and Flavonoids Biosynthesis of Buckwheat
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摘要 以荞麦无菌苗胚轴作为外植体诱导愈伤组织,研究了光照、蔗糖、L-苯丙氨酸和植物激素(2,4-D、NAA、6-BA)等因素对荞麦愈伤组织生长及黄酮合成的影响,为荞麦大规模细胞培养和工业化生产黄酮类化合物奠定基础。结果表明:光照对荞麦愈伤组织生长没有明显的影响,但光照和黑暗交替处理能促进愈伤组织黄酮合成;蔗糖质量浓度为4%,荞麦的愈伤组织生物量、黄酮含量和产量达到最高;L-苯丙氨酸能促进荞麦愈伤组织黄酮合成,但对愈伤组织生长有一定的抑制作用,L-苯丙氨酸浓度为150μmol.L-1,黄酮产量达到最高;在一定浓度范围内,3种植物激素都能促进荞麦愈伤组织生长和黄酮合成,但2,4-D和6-BA的效果要优于NAA,但2,4-D的效果最佳。促进荞麦愈伤组织生长和黄酮积累最佳的激素配比组合为1.5mg.L-12,4-D+0.6mg.L-16-BA。 Buckwheat callus induced from the hypocotyl of its sterile seedling were cultivated in different light period treatment, different concentration of sucrose, L-phenylalanine and phytohormone to study the influence of different culture conditions on the growth of buckwheat callus and their flavonoids synthesis, those laid to a foundation for mass buckwheat cell culture and industrialized mass production of flavones. The result showed that : Light period treatment had no obvious effect on biological yield of buckwheat callus, but the light and dark processing alternately could promote the flavonoid synthesis; When the sucrose concentration was 4%, the biological yield of buckwheat callus, content and biological yield of flavones was reached maximum value; L-phenylalanine could promote the flavonoid synthesis in buckwheat callus,but inhibit the growth of buckwheat callus, when the L-phenylalanine concentration was 150μmol. L-1, biological yield of flavones was reached maximum value; In certain range of concentration, three kinds of phytohormone could promote the buckwheat callus growth and flavonoid synthesis, the effect of 2,4-D and 6-BA was superior to NAA,the effect of 2,4-D was the best,the best phytohormone mixture ratio of promoting biological yield of buckwheat callus and flavonoids synthesis was 1. 5mg. L-1 2,4-D +0.6mg.L-1 6-BA.
出处 《核农学报》 CAS CSCD 北大核心 2013年第5期591-597,共7页 Journal of Nuclear Agricultural Sciences
基金 国家自然科学基金(31071472) 唐仲英基金(A212020923)
关键词 荞麦 培养条件 愈伤组织 黄酮 Buckwheat Culture condition Callus Flavones
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