维生素A对小鼠胚胎Hox3.5基因表达的影响

Effects of vitamin A on Hox3.5 gene expression in mouse embryo

为观察维生素 A(VA)缺乏及再补充对小鼠胚胎 Hox C4(3.5 )基因表达的影响 ,将初断乳的 6 0只昆明种雌鼠随机分为 4组 :正常对照组 (N)饲含 VA40 0 0 IU/ kg饲料 ,其他 3组 (A、B、C组 )在交配前饲缺乏 VA饲料 ,B和 C组分别在妊娠的第 0和 7天饲含 VA 10 0 0 0 IU / kg的饲料 ,A组饲料不变。30只雄鼠饲以普通饲料 ,在饲养的第 16周按雌 :雄 =2 :1合笼交配。在妊娠第 12天脱颈椎处死雌鼠 ,立即剖腹取出胎鼠 ,置于液氮中速冻后于 - 70℃冰箱保存。用地高辛标记的探针 ,采用原位杂交方法探测小鼠胚胎 Hox C4(3.5 )基因 m RNA含量。结果显示 ,A组 m RNA的含量明显减少 ;B组 m RNA含量与 N组无差异 ;C组 m RNA含量虽比 A组增加 ,但仍低于 N组。结果提示 ,VA可能通过调控 Hox基因的表达来影响胚胎发育。

In order to investigate the effects of vitamin A(VA)deficiency and VA supplementation on Hox 3 5 gene expression in mouse embryo,60 Kunming weaning female mice were divided into four groups.The normal control group(N) was fed diet containing VA 4 000 IU/kg.The other 3 groups were fed VA deficient diet(VA 0 IU/kg diet)for 16 weeks and then make all mice mating with normal male mice.Group A was fed VA deficient diet all through the experiment.Group B and group C were fed diet supplemented with VA (VA 10 000IU/kg)from the day zero and from the 7th day after conception respectively.On the 12th day of gestation,6 pregnant mice in each group were killed and the embryos were taken immediately.In situ hybridization was used to determine the contents of mRNA of Hox 3 5 gene in mice embryos by digoxin labeled cDNA probes.The results revealed that the contents of Hox 3 5 gene mRNA in the embryos of group A and C decreased significantly compared from group N and B,and that in group B had no difference from group N.The mRNA contents of group C were higher compared with group A,but still was lower than that of group N.It was concluded that vitamin A might influence the development of embryos by regulating the expression of homeobox gene.