摘要
对蔓茎堇菜细胞悬浮培养的条件进行了选择 ,并测定悬浮培养的生长动态 .结果表明 :蔓茎堇菜细胞悬浮培养的最适接种量为 2 .0~ 3.5g (5 0mL) ;最适培养基为MS +2 .0mg/L 2 ,4 D +1.0mg/LZT +30 0mg/LLH+2 0 0g/L蔗糖 (或葡萄糖 ) ,pH 5 .8;细胞悬浮培养一个周期过程中细胞数目增长的动态基本上是一条S型曲线 ,对数生长期明显 ;细胞鲜重 (mFW)和细胞干重 (mDW)则与细胞数目不同 ,没有明显的对数期 ,基本上呈逐步上升的状态 ;蔓茎堇菜细胞悬浮培养中还存在细胞增殖与pH升降之间的相关性 .图 3表 2参
The conditions of cell suspension culture and growth kinetics of Viola diffusa Ging were studied. The results showed that the fittest inoculating quantity of Viola diffusa Ging in cell suspension culture was 2.0~3.5 g (50 mL). The fittest culture media were MS+2.0 mg/L ?2,4 D+1.0 mg/L ZT+300 mg/L LH+200/L sucrose (or glucose), with pH 5.8. The cell number development of Viola diffusa Ging during a culture course was similar to a “S” curve with apparent logarithmic phase. Contrast to the cell number, fresh weight ( m FW ) and dry weight ( m DW ) had no apparent logarithmic phase, but a gradually rising tendency appeared. There was also a relationship between cell multiplication and pH during the suspension cell culture of Viola diffusa Ging. Fig 3, Tab 2, Ref 12
出处
《应用与环境生物学报》
CAS
CSCD
2000年第1期43-47,共5页
Chinese Journal of Applied and Environmental Biology
基金
福建省教委科学基金资助项目!(编号JA97145 )
关键词
蔓茎堇菜
细胞悬浮培养
黄酮
培养基
药用作物
Viola diffusa Ging
cell suspension culture
callus culture
cell development