CD11b及CD64在早产儿感染早期诊断中意义

Significance of CD11b and CD64 in early diagnosis of infections of premature infants

目的探讨CD11b与CD64在早产儿感染早期诊断中的价值。方法胎龄≤36周早产儿60例,感染组29例与非感染组31例,采用流式细胞仪检测2组中性粒细胞CD11b、中性粒细胞及单核细胞CD64的平均荧光强度,并进行比较。结果感染组早产儿中性粒细胞CD11b、中性粒细胞CD64、单核细胞CD64的表达水平明显高于非感染组,差异有统计学意义(P<0.05);中性粒细胞CD11b、中性粒细胞CD64及单核细胞CD64在早产儿早期感染的最佳临床诊断界值分别为1 151、1 013、4 340,敏感度分别为69%、72%、76%,特异性分别为65%、74%、71%,阳性预测值分别为65%、72%、71%,阴性预测值分别为69%、74%、76%。结论早产儿早期感染时中性粒细胞CD11b、中性粒细胞CD64、单核细胞CD64平均荧光强度均升高,其中CD64的表达明显升高,可作为早期诊断早产儿感染的实验室依据。

Objective To investigate the value of CDllb and CD64 expression in peripheral blood to the early diagnosis of infections of premature infection. Methods Sixty premature infants with gestational age 436 weeks were divided into infection group （n=29） and non-infection group （n= 31）. The expressions of neutrophil CDllb, neutrophil CD64 and monocyte CD64 were detected by using flow eytometry, and were compared between two groups. Results The mean fluorescence intensity expression of neutrophil CDllb, neutrophil CD64 and monocyte CD64 were significantly higher in infection group than those in non-infection group （P〈0.05）. The optimal cut-off values of neutrophil CDllb, neutrophil CD64 and monocyte CD64 for diagnosing infection were 1 151, 1 013 and 4 340 respectively. The sensitivities of neutrophil CDllb, neutrophil CD64 and monocyte CD64 were 69%, 72% and 76%, the specificities were 65%, 74% and 71%, the positive predictive values were 65 %, 72% and 71%, and the negative predictive values were 69%, 74% and 76% respectively. Conclusion Mean fluorescence intensities of CDllb and CD64 on neutrophil, and CD64 on monocyte increase during early infection in premature infants, and the increase of CD64 expression is the most obvious, which could be used as one of the laboratory markers for early diagnosis of infections in premature infants .