镇肝熄风汤对实验性心肌缺血大鼠的治疗作用及其机制探讨

Therapeutic effects and mechanisms of Zhengan Xifeng decoction on the experimental rats with myocardial ischemia

目的:观察镇肝熄风汤对大鼠实验性心肌缺血损伤的保护作用。方法:采用结扎冠脉左前降支的方法复制大鼠心肌缺血模型,将造模成功的大鼠随机均分为模型组、阳性对照组、镇肝熄风汤低、中、高剂量组,每组10只,另取假手术组10只,连续灌胃28 d,分别监测各组大鼠手术前及手术后即刻、1、3、7、10、14、21、28 d的心电图,并测定给药28 d后的心肌梗死率及血清中NO、ET的含量。结果:镇肝熄风汤各剂量组大鼠心电图S-T段的抬高程度均显著下降,心肌梗死范围明显缩小,血清NO水平明显升高,ET含量明显降低。结论:镇肝熄风汤能有效改善大鼠实验性心肌缺血损伤,其作用可能与改善血管内皮活性物质释放,保护血管内皮功能有关。

Objective ：To investigate the effects of Zhengan Xifeng decoction on the experimental rats with myocardial ischemia. Methods ： Animal models of myocardial ischemia were prepared by ligation of the coronary artery at left anterior descending. The competent models were randomized into model group, positive controls, groups of administration of Zhengan Xifeng decoction in dosage of low, medium and high besides a sham operation group（ n = 10 for each group）. Intragastric administration of the drug was carried out for consecutive 28 days. Electrocardiograms （ECG） for each group of animals were measured before surgery, immediately after surgery, and on day 1,3,7,10,14,21 and 28 after operation, respective- ly. The scope of myocardial infarction, the content of nitric oxide （NO） and endothelin （ET） in the serum of each rat were determined after 28 days of the drug administration. Results ： Significantly decreased S-T in ECG,reduced extent of infarction, elevated NO level yet decreased ET content were found in a rats treated with different dosage of Zhengan Xifeng decoction. Conclusion：Zhengan Xifeng decoction can effectively prevent the rats from myocardial ischemic injury. The potential protective mechanism may be associated with the release of certain active compounds capable of protecting the function of the vascular endothelium.