H-FABP不同基因型对滇南小耳猪脂肪分解代谢相关基因表达的影响

Expression of Lipid Catabolism Genes in Diannan Small-ear Pigs with Different H-FABP Genotypes

本研究旨在探讨不同H-FABP基因型滇南小耳猪肌内脂肪细胞脂类代谢相关基因的表达及其与肌内脂肪细胞甘油三酯(Triglycerol,TG)含量的相关性。本研究利用试剂盒测定肌内脂肪细胞TG含量,采用RT-qPCR检测肌内脂肪细胞脂类分解代谢基因mRNA表达水平。结果显示:HH基因型个体的脂类分解代谢相关基因肉碱脂酰转移酶1(Carnitine palmitoyl transferase 1,CPT-1)、脂蛋白酯酶(Lipoprotein lipase,LPL)和过氧化物酶体增殖物激活受体γ(Preoxisome proliferator-activated receptorγ,PPARγ)3种基因mRNA表达水平均显著高于hh基因型个体(P<0.05);且CPT-1、LPL、PPARγ基因mRNA的表达量与肌内脂肪细胞TG含量呈正相关。不同H-FABP基因型影响滇南小耳猪肌内脂肪细胞中脂肪分解代谢相关基因的表达,HH基因型猪脂肪分解代谢相关基因的表达量较高,可能脂类代谢活动更强,从而相对增加了肌内脂肪沉积。

This study aimed to investigate the expression of the lipid metabolism related genes, and the association between the lipid metabolism related genes and triglyceride（TG） content in in- tramuscular fat cells of Diannan Small-ear pigs with different H-FABP genotypes. The kit was used to determine the triglyceride（TG） content and to investigate the level of mRNA expression of lipid catabolism genes by reverse transcription quantitative real-time PCR（RT-qPCR）. The re- sults showed that significantly higher expression levels of earnitine palmitoyl transferase 1 （CPT-1）, lipo- protein lipase（LPL） and preoxisome proliferator-activated receptor y（PPARy） mRNA were found in the HH genotype individuals comparing with that in hh genotype individuals（P〈0.05）. The expression levels of CPT-1, LPL, PPAR y genes and TG content in intramuscular adipoeytes were positively correlated. The expression of fat catabolism related genes in Diannan Small-ear pigs with different H-FABP genotypes is discrepant, the expression level of the pig with HH genotype is higher. As the lipid metabolic activity is stronger, this may increase intramuscular fat deposition relatively.