CDV免疫蛋鸡血清抗体与卵黄抗体的消长规律及其相关性分析

Dynamics and Correlation Analysis of Serum and Egg Yolk Antibodies of Laying Hens Immuned by CDV

为了解产蛋鸡对犬瘟热病毒杭原的免疫反应及其血清抗体和卵黄抗体的消长规律及相关性,为制备卵黄抗体提供依据,将犬瘟热病毒接种Vero细胞和DF1细胞进行传代并测定其病毒滴度,选取细胞病变出现早,病毒滴度高的Vero细胞毒作为接种病毒抗原免疫蛋鸡。通过每10d进行蛋鸡免疫一次,四免后每30d加强免疫一次的方法进行免疫。免疫前和免疫后每10d采血分离血清和每5d收集鸡蛋提取卵黄抗体,用琼脂扩散法和间接ELISA法测定其抗体水平。结果显示,血清抗体比卵黄抗体的滴度高,两者间呈正相关性,卵黄抗体出现较血清抗体迟3～5d,卵黄抗体在三免后3～5d就已达到较高水平,此时即可开始收集鸡蛋制备卵黄抗体。

To study the immune response, dynamics and its correlation analysis of the serum and egg yolk antibody in laying hens against CDV antigen, and to provide the basis for the preparation of egg yolk antibody, Vero cell and DF1 cell cultures were inoculated with CDV to detect the virus titer. Select the Vero cytotoxic strain with earlier cytopathic and high virus titers as inoculated virus antigen to immunize laying hens. immunize the laying hens once every 10 days, for four times successively, then strengthen the immune every 30 days. Blood serum was separated every 10 days and egg yolk antibody was collected every 5 days before and after immunization, the antibody levels were determined by agar diffusion and the indi- rect ELISA. The results showed that serum antibody titer was higher than yolk antibody and both were positively correlated the appearance of yolk antibody was 3 to 5 days later than serum antibody. Egg yolk antibody has reached the highest level in 3 to 5 days after three immunities,and this is the righttime to col- lect eggs and to prepare yolk antibody.