芪莲舒痞方对CAG大鼠胃黏膜血流量及病理形态影响的实验研究

Experimental Research of the Impacts of Qilian Shupi Formula on Gastric Mucosal Blood flows and Pathological Morphology for CAG Rats

目的通过随机、对照动物实验,观察芪莲舒痞(QLSP)方对慢性萎缩性胃炎(chronicatrophic gastritis,CAG)癌前病变大鼠胃黏膜病理形态、胃黏膜血流量及胃黏膜厚度的影响,探讨芪莲舒痞方的作用机理。方法将大鼠120只按体重随机分组,分出正常组20只,其余大鼠采用"MNNG+雷尼替丁+乙醇+饥饱失调"的综合造模方法复制CAG癌前病变Wistar大鼠模型,通过随机抽样病理检测确定造模成功后,将造模成功大鼠随机分为模型组、胃复春组、芪莲舒痞大、中、小剂量组各15只,并分别给予药物干预后,观察胃黏膜病理变化,检测胃黏膜血流量及胃黏膜厚度。结果芪莲舒痞方能降低大鼠CAG癌前病变的发生率,增加CAG癌前病变大鼠胃黏膜血流量和胃黏膜厚度。结论芪莲舒痞方是治疗CAG癌前病变的有效复方,能明显增加CAG大鼠胃黏膜血流量及胃黏膜厚度,可能是其作用机制之一。

Objective To observe the impacts of qilian shupi formula on the gastric mucosal pathological morphology, blood flow and thickness for the rats with chronic atrophic gastritis（CAG） and precancerous lesions by the randomized and controlled animal experiment. Methods 120 rats were randomized into groups according to the body weight. 20 rats were divided into a normal group. For the rest rats, the complex modeling method,＂ MNNG ＋ ranitidine ＋ ethanol ＋ appetite maladjustment＂ was adopted to duplicate Wistar rat models of CAG precancerous lesion. Determined by the random sampling pathological detection, the rats of successful modeling were randomized into a model group, a weifuchun group and the large - dose, middle - dose and small - dose qilian shupi formula groups, 15 cases in each one. After corresponding medicinal intervention,the gastric mueosal pathological change was observed and the gastric mucosal blood flow and thickness were detected. Results Qilian shnpi formula reduced the incidence of CAG precancerous lesion for the rats,increased the gastric mucosal blood flow and thickness for the rats with CAG precancerous lesion. Conclusion Qilian shupi formula is the effective compound for the treatment of CAG precancerous lesion and it can apparently increase gastric mucosal blood flow and thickness for CAG rats, which is probably one of the mechanisms on the effect.