摘要
目的探讨小肠黏膜下层(small intestinal submucosa,SIS)作为盖髓材料的可行性。方法分别以SIS、聚乳酸(polylactic acid,PLA)医用膜为细胞支架与人牙髓细胞复合培养,测定2组细胞粘附率、细胞增殖指数,并通过比较2组细胞的碱性磷酸酶活性水平和钙结节形成的大小,研究各组牙髓细胞的矿化能力。结果复合培养24 h时SIS组的细胞粘附率为64.36%,高于PLA组(P<0.05),且复合培养3、5、7 d时SIS组的细胞增殖指数分别为23.0、18.9、17.9,均高于PLA组(P<0.05)。SIS组细胞的碱性磷酸酶活性水平在7、14、21 d均高于PLA组(P<0.05),并且28 d时SIS组形成钙结节,PLA组未形成明显的钙结节。结论 SIS有良好的生物相容性,在体外能促进牙髓细胞增殖、矿化,有望成为一种新型的盖髓材料。
Objectvies To investigate the feasibility of using small intestinal sub mucosa(SIS) as pulp capping agent.Methods SIS and medical films of PLA were used as cytoskeletons and compositely cultured with human dental pulp cells(HDPCs).The rate of cells adhesion and proliferation index were measured.Compare the levels of ALP activity and the sizes of calcium noduls in the two groups to evaluate the mineralization capacity of HDPCs.Results The rate of cell adhesion in SIS group at 24h was 64.36%,which was significantly higher than that of PLA group(P〈0.05).The cell proliferation index in SIS group after 3,5,7 d was 23.0,18.9,17.9 separately,which was significantly higher than that in PLA group(P〈0.05).The levels of ALP activity in SIS group were higher than those in PLA group on 7,14,21 d(P〈0.05).The calcium nodules appeared in SIS group on 28d,which did not appear in PLA group.Conclusions SIS is of good biocompatibility and can promote proliferation and mineralization of human dental pulp cells in vitro.Therefore,SIS is a new feasible pulp capping material.
出处
《口腔医学》
CAS
2013年第6期376-379,共4页
Stomatology
基金
上海市科学技术委员会基础重点项目资助(12JC1407301)
上海市自然基金青年项目资助(12ZR1447200)
上海市第六人民医院院级课题项目资助(院内-1517)
关键词
小肠黏膜下层
牙髓细胞
盖髓材料
活髓保存
small intestinal submucosa
pulp cells
pulp capping agents
vital pulp preservation