利用黄瓜做生物反应器表达人胰岛素的研究

Study on Expression of Human Insulin by Cucumber Bio-reactor

[目的]研究利用黄瓜作为生物反应器时人胰岛素的表达情况。[方法]通过农杆菌介导法将修饰过的人胰岛素基因(hINS)转入黄瓜,构建表达人胰岛素的生物反应器,再利用PCR和RT-PCR方法检测hINS的表达情况,最后利用NOD小鼠饲喂试验检测表达蛋白的疗效。[结果]研究成功构建了能够稳定表达人胰岛素的生物反应器,目的蛋白表达量占其总可溶性蛋白含量的0.042%～0.190%;NOD小鼠饲喂试验证实,表达产物对NOD小鼠的糖尿病防治具有显著疗效。[结论]该研究表明利用转基因植物作为生物反应器生产医用蛋白和药品具有理论可行性和巨大的利用价值。

[Objective] The paper was to study the expression of human insulin by using cucumber as bio-reactor.[Method] The modified human insulin gene（hINS） was transported into cucumber by Agrobacterium mediated method,and the bio-reactor which could express human insulin was constructed.The expression of hINS was detected by the PCR and RT-PCR methods,and the effect of the expressed protein was determined by NOD mice feeding experiments finally.[Result] The bio-reactor with stable expression of human insulin had been constructed successfully,and the expression protein content was 0.042%-0.190% of the total soluble protein content;the NOD mice feeding experiments confirmed that,the expression product of cucumber bio-reactor had a significant effect for the prevention and treatment of diabetes.[Conclusion]The study showed using transgenic plants as bio-reactor to produce medical proteins and drugs had a great significance not only in theory but also in practice.