摘要
建立了梯度洗脱高效液相色谱法测定注射用硫酸头孢匹罗的有关物质。采用Kromasil C18色谱柱,以pH 7.0磷酸盐缓冲液-乙腈为流动相,梯度洗脱,检测波长265 nm。结果显示头孢匹罗与有关物质分离良好。头孢匹罗、7-氨基头孢烷酸和2,3-环戊烯并吡啶分别在1～16μg/ml、0.5～10μg/ml和1～10μg/ml范围内线性关系良好。
A gradient elution HPLC method was established for the determination of the related substances in cefpirome sulfate for injection. A Kromasil C18 column was used with the mobile phase of phosphate buffer （pH 7.0）-acetonitrile by gradient elution at the detection wavelength of 265 nm. The results indicated that cefpirome and the related substances could be separated well. The calibration curves of cefpirome, 7-ACA and 2,3-cyclopentenopyridine （2,3-CYCPP） were linear in the concentration ranges of 1 - 16 μg/ml, 0.5 - 10 μg/ml and 1 - 10 μg/ml, respectively.
出处
《中国医药工业杂志》
CAS
CSCD
北大核心
2013年第7期716-719,共4页
Chinese Journal of Pharmaceuticals
